Immunoblotting of soluble antigens in Paracoccidioides brasiliensis culture

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2014-03-01
Autores
Carlos, Nilson de Jesus
Pinto, Debora Alves
Ono, Mario Augusto
Venancio, Emerson Jose
Camargo, Zoilo Pires de [UNIFESP]
Sano, Ayako
Itano, Eiko Nakagawa
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This study investigated the major soluble antigens produced by Paracoccidioides brasiliensis (Pb339) cultured in solid Sabouraud (pH 5.6 and 8.5), Sabouraud plus brain heart infusion and liquid tomato juice-enriched complex medium media at intervals of 3 days over 30 days by immunoblotting and concluded that, to optimize the source of each antigen, both time and growth conditions should be considered.
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Microbiology and Immunology. Hoboken: Wiley-Blackwell, v. 58, n. 3, p. 212-214, 2014.