Strain-specific protective immunity following vaccination against experimental Trypanosoma cruzi infection

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dc.contributor.authorHaolla, Filipe Augusto Bettencourt [UNIFESP]
dc.contributor.authorClaser, Carla [UNIFESP]
dc.contributor.authorAlencar, Bruna Cunha Gondim de [UNIFESP]
dc.contributor.authorTzelepis, Fanny [UNIFESP]
dc.contributor.authorVasconcelos, Jose Ronnie Carvalho de [UNIFESP]
dc.contributor.authorOliveira, Gabriel de
dc.contributor.authorSilverio, Jaline Coutinho
dc.contributor.authorMachado, Alexandre Vieira
dc.contributor.authorLannes-Vieira, Joseli
dc.contributor.authorBruna-Romero, Oscar
dc.contributor.authorGazzinelli, Ricardo Tostes
dc.contributor.authorSantos, Ricardo Ribeiro dos
dc.contributor.authorSoares, Milena Botelho Pereira
dc.contributor.authorRodrigues, Mauricio Martins [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionFiocruz MS
dc.contributor.institutionUniversidade Federal de Minas Gerais (UFMG)
dc.contributor.institutionUniv Massachusetts
dc.contributor.institutionHosp Sao Rafael
dc.date.accessioned2016-01-24T13:58:45Z
dc.date.available2016-01-24T13:58:45Z
dc.date.issued2009-09-18
dc.description.abstractImmunisation with Amastigote Surface Protein 2 (asp-2) and trans-sialidase (ts) genes induces protective immunity in highly susceptible A/Sn mice, against infection with parasites of the Y strain of Trypanosoma cruzi. Based on immunological and biological strain variations in T cruzi parasites, our goal was to validate our vaccination results using different parasite strains. Due to the importance of the CD8(+) T cells in protective immunity, we initially determined which strains expressed the immunodominant H-2K(k)-restricted epitope TEWETGQI. We tested eight strains, four of which elicited immune responses to this epitope (Y, G, Colombian and Colombia). We selected the Colombian and Colombia strains for our studies. A/Sn mice were immunised with different regimens using both T. cruzi genes (asp-2 and ts) simultaneously and subsequently challenged with blood trypomastigotes. Immune responses before the challenge were confirmed by the presence of specific antibodies and peptide-specific T cells. Genetic vaccination did not confer protective immunity against acute infection with a lethal dose of the Colombian strain. in contrast, we observed a drastic reduction in parasitemia and a significant increase in survival, following challenge with an otherwise lethal dose of the Colombia strain. in many surviving animals with late-stage chronic infection, we observed alterations in the heart's electrical conductivity, compared to naive mice. in summary, we concluded that immunity against T cruzi antigens, similar to viruses and bacteria, may be strain-specific and have a negative impact on vaccine development. (c) 2009 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, CINTERGEN, BR-04044010 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04044010 São Paulo, Brazil
dc.description.affiliationFiocruz MS, Inst Oswaldo Cruz, Biol Lab, BR-21045900 Rio de Janeiro, Brazil
dc.description.affiliationFiocruz MS, Inst Oswaldo Cruz, Lab Biol Interacoes, BR-21045900 Rio de Janeiro, Brazil
dc.description.affiliationFiocruz MS, Ctr Pesquisas Rene Rachou, BR-30190002 Belo Horizonte, MG, Brazil
dc.description.affiliationUniv Fed Minas Gerais, Inst Ciencias Biol, Dept Microbiol, BR-31270901 Belo Horizonte, MG, Brazil
dc.description.affiliationUniv Fed Minas Gerais, Inst Ciencias Biol, Dept Bioquim & Imunol, BR-31270901 Belo Horizonte, MG, Brazil
dc.description.affiliationUniv Massachusetts, Sch Med, Dept Med, Div Infect Dis & Immunol, Worcester, MA 01655 USA
dc.description.affiliationFiocruz MS, Ctr Pesquisas Goncalo Moniz, BR-40295001 Salvador, BA, Brazil
dc.description.affiliationHosp Sao Rafael, BR-41253190 Salvador, BA, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, CINTERGEN, BR-04044010 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04044010 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)pt
dc.description.sponsorshipIDFAPESP: 2006/1983-4pt
dc.description.sponsorshipIDCNPq: 420067/2005-1pt
dc.description.sponsorshipIDFAPEMIG: EDT 24.000pt
dc.format.extent5644-5653
dc.identifierhttps://dx.doi.org/10.1016/j.vaccine.2009.07.013
dc.identifier.citationVaccine. Oxford: Elsevier B.V., v. 27, n. 41, p. 5644-5653, 2009.
dc.identifier.doi10.1016/j.vaccine.2009.07.013
dc.identifier.issn0264-410X
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/31818
dc.identifier.wosWOS:000270070400015
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofVaccine
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectTrypanosoma cruzien
dc.subjectDNA vaccineen
dc.subjectAdenovirus vaccineen
dc.subjectCD8en
dc.titleStrain-specific protective immunity following vaccination against experimental Trypanosoma cruzi infectionen
dc.typeinfo:eu-repo/semantics/article
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