Proteomic analysis of seminal plasma in adolescents with and without varicocele

dc.contributor.authorZylbersztejn, Daniel Suslik [UNIFESP]
dc.contributor.authorAndreoni, Cassio [UNIFESP]
dc.contributor.authorDel Giudice, Paula Toni [UNIFESP]
dc.contributor.authorSpaine, Deborah Montagnini [UNIFESP]
dc.contributor.authorBorsari, Luciana
dc.contributor.authorSouza, Gustavo H. M. F.
dc.contributor.authorBertolla, Ricardo Pimenta [UNIFESP]
dc.contributor.authorFraietta, Renato [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionSch Natl Serv Ind Training
dc.contributor.institutionWaters Corp
dc.date.accessioned2016-01-24T14:28:11Z
dc.date.available2016-01-24T14:28:11Z
dc.date.issued2013-01-01
dc.description.abstractObjective: To compare proteomic profiles of seminal plasma from adolescents with varicocele and changes in semen quality with the plasma from adolescents with varicocele without seminal changes and from adolescents without varicocele.Design: Observational study.Setting: Patients in an academic research environment.Patient(s): Adolescents without varicocele (control group), adolescents with varicocele and normal semen quality (VNS group), adolescents with varicocele and abnormal semen quality (VAS group).Intervention(s): Two semen collections at 1-week interval. Protein separation by two-dimensional protein electrophoresis, analysis by gel densitometry, and identification by mass spectrometry.Main Outcome Measure(s): Overexpressed proteins in each group, observed by increased densitometric signal in gels, and exclusively identified proteins in each group.Result(s): No differences were observed among the three groups regarding clinical parameters. in semen analysis, the VAS group presented lower sperm concentration, motility, and morphology compared with the VNS and control groups. Forty-seven protein spots of interest were submitted to mass spectrometry identification. Apoptosis regulation proteins were overexpressed in the VAS group, whereas spermatogenesis proteins were overexpressed in the VNS group. Controls presented proteins related to homeostasis.Conclusion(s): Changes in the proteomic profile of adolescents with varicocele and normal semen parameters (VNS group) indicate that normal semen analysis may not reflect alterations in proteins in seminal plasma. Implementation of proteomics will help characterize proteins identified in seminal plasma and will facilitate detection of new proteins associated with spermatogenesis and sperm function. (Fertil Steril (R) 2013;99:92-8. (C) 2013 by American Society for Reproductive Medicine.)en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Surg, Div Urol, Human Reprod Sect, BR-04038032 São Paulo, Brazil
dc.description.affiliationSch Natl Serv Ind Training, São Paulo, Brazil
dc.description.affiliationWaters Corp, MS Applicat & Dev Lab, Alphaville, Barueri, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Surg, Div Urol, Human Reprod Sect, BR-04038032 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent92-98
dc.identifierhttp://dx.doi.org/10.1016/j.fertnstert.2012.08.048
dc.identifier.citationFertility and Sterility. New York: Elsevier B.V., v. 99, n. 1, p. 92-98, 2013.
dc.identifier.doi10.1016/j.fertnstert.2012.08.048
dc.identifier.issn0015-0282
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/35645
dc.identifier.wosWOS:000313044400021
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofFertility and Sterility
dc.rightsAcesso aberto
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectVaricoceleen
dc.subjectadolescenten
dc.subjectproteomicsen
dc.subjectsemen analysisen
dc.subjectseminal plasmaen
dc.titleProteomic analysis of seminal plasma in adolescents with and without varicoceleen
dc.typeArtigo
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