Leucine-rich amelogenin peptide: A candidate signaling molecule during cementogenesis

dc.contributor.authorBoabaid, F.
dc.contributor.authorGibson, C. W.
dc.contributor.authorKuehl, M. A.
dc.contributor.authorBerry, J. E.
dc.contributor.authorSnead, M. L.
dc.contributor.authorNociti, F. H.
dc.contributor.authorKatchburian, E. [UNIFESP]
dc.contributor.authorSomerman, M. J.
dc.contributor.institutionUniv Washington
dc.contributor.institutionUniv Michigan
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Penn
dc.contributor.institutionUniv So Calif
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.description.abstractBackground: Cementum is a critical mineralized tissue; however, control of its formation remains undefined. One hypothesis is that enamel matrix proteins/peptides secreted by ameloblasts and/or epithelial rest cells contribute to the control of cementum formation via epithelial-mesenchymal interactions. Here, we focused on determining whether or not leucine-rich amelogenin peptide (LRAP), translated from an alternatively spliced amelogenin RNA, altered cementoblast behavior.Methods: Immortalized murine cementoblasts (OCCM-30) were exposed to LRAP and evaluated for: 1) proliferative activity; 2) gene expression using Northern blot for Cbfa1 (core binding factor alpha-1); OCN (osteocalcin), OPN (osteopontin), and real-time reverse transcription-polymerase chain reaction (RT-PCR) for OPG (osteoprotegerin); and RANKL (receptor activator of NF-kappaB ligand); 3) signaling pathway using inhibitors of PKA (THFA), PKC (GF109203X), and MAPK (UO126); and 4) mineralization evaluated by von Kossa and Alizarin-red.Results: LRAP had no effect on cell proliferation up to 6 days, with a decrease in cell growth observed at the highest dose by 9 days versus untreated cells. LRAP down regulated OCN and up regulated OPN in a dose- and time-response fashion, and inhibited the capacity of mineral nodule formation. Transcripts for OPG were increased in LRAP-treated cells compared to control, but RANKL mRNA levels were not affected. Core binding factor alpha (Cbfa) mRNA, expressed constitutively, was not affected by LRAP. Signaling pathway assays suggested involvement of the MAPK pathway, since the addition of the MAPK inhibitor suppressed OPN expression in LRAP-treated cells.Conclusion: Leucine-rich amelogenin peptide appears to have a direct effect on cementoblast activity that may prove significant during development as well as in regeneration of periodontal tissues.en
dc.description.affiliationUniv Washington, Hlth Sci Ctr, Sch Dent, Dept Periodont, Seattle, WA 98195 USA
dc.description.affiliationUniv Michigan, Sch Dent, Dept Periodont Prevent Geriatr, Ann Arbor, MI 48109 USA
dc.description.affiliationUniversidade Federal de São Paulo, Sch Med, Dept Morphol, São Paulo, Brazil
dc.description.affiliationUniv Penn, Sch Dent Med, Dept Anat & Cell Biol, Philadelphia, PA 19104 USA
dc.description.affiliationUniv So Calif, Sch Dent, Ctr Craniofacial Mol Biol, Los Angeles, CA 90089 USA
dc.description.affiliationUniv Campinas, Sch Dent Piracicaba, Dept Periodont, Piracicaba, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Sch Med, Dept Morphol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.identifier.citationJournal of Periodontology. Chicago: Amer Acad Periodontology, v. 75, n. 8, p. 1126-1136, 2004.
dc.publisherAmer Acad Periodontology
dc.relation.ispartofJournal of Periodontology
dc.rightsAcesso restrito
dc.subjectdental cementumen
dc.subjectleucine-rich amelogeninen
dc.subjectperiodontal regenerationen
dc.titleLeucine-rich amelogenin peptide: A candidate signaling molecule during cementogenesisen