Inhibition of Leishmania (Leishmania) amazonensis growth and infectivity by aureobasidin A

dc.contributor.authorTanaka, Ameria K. [UNIFESP]
dc.contributor.authorValero, Valderez B [UNIFESP]
dc.contributor.authorTakahashi, Helio K. [UNIFESP]
dc.contributor.authorStraus, Anita H. [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:41:53Z
dc.date.available2016-01-24T12:41:53Z
dc.date.issued2007-03-01
dc.description.abstractObjectives: To study the effect of aureobasidin A, an inhibitor of inositol phosphorylceramide (IPC) synthase, on Leishmania growth and infectivity.Methods: Effects of aureobasidin A were determined for: (i) promastigote growth in axenic culture; (ii) promastigote infectivity in macrophage monolayers; (iii) development of footpad lesions in BALB/c mice; (iv) differentiation of amastigotes into promastigotes.Results: Aureobasidin A (20 mu M) inhibited 90% of Leishmania (Leishmania) amazonensis promastigote growth in axenic culture, but the parasites remained viable, i.e. growth curves returned to normal after aureobasidin A was removed from culture medium. the aureobasidin A IC50 was determined by MTT assay as 4.1 mu M for L. (L.) amazonensis promastigotes, 12.6 mu M for Leishmania (Leishmania) major and 13.7 mu M for Leishmania (Viannia) braziliensis. There was a significant delay in infection when L. (L.) amazonensis promastigotes pre-treated with aureobasidin A were inoculated into BALB/c mouse footpads. When aureobasidin A was added to cultured macrophages infected with amastigotes, the number of infected macrophages was reduced by >90%.Conclusions: Aureobasidin A is an interesting pharmacological tool to investigate the effect of lipid metabolism inhibition in Leishmania spp.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Biochem, Escola Paulista Med, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Biochem, Escola Paulista Med, BR-04023900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent487-492
dc.identifierhttp://dx.doi.org/10.1093/jac/dkl518
dc.identifier.citationJournal of Antimicrobial Chemotherapy. Oxford: Oxford Univ Press, v. 59, n. 3, p. 487-492, 2007.
dc.identifier.doi10.1093/jac/dkl518
dc.identifier.issn0305-7453
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/29516
dc.identifier.wosWOS:000245628200019
dc.language.isoeng
dc.publisherOxford Univ Press
dc.relation.ispartofJournal of Antimicrobial Chemotherapy
dc.rightsAcesso aberto
dc.rights.licensehttp://www.oxfordjournals.org/access_purchase/self-archiving_policyb.html
dc.subjectanti-Leishmaniaen
dc.subjectsphingolipidsen
dc.subjectamastigotesen
dc.titleInhibition of Leishmania (Leishmania) amazonensis growth and infectivity by aureobasidin Aen
dc.typeArtigo
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