Single embryo and oocyte lipid fingerprinting by mass spectrometry

Página do item simplificado
dc.contributor.authorFerreira, Christina R.
dc.contributor.authorSaraiva, Sergio A.
dc.contributor.authorCatharino, Rodrigo R.
dc.contributor.authorGarcia, Jerusa S.
dc.contributor.authorGozzo, Fabio C.
dc.contributor.authorSanvido, Gustavo B.
dc.contributor.authorSantos, Luiz Fernando A.
dc.contributor.authorLo Turco, Edson G. [UNIFESP]
dc.contributor.authorPontes, Jose Henrique F.
dc.contributor.authorBasso, Andrea C.
dc.contributor.authorBertolla, Ricardo P. [UNIFESP]
dc.contributor.authorSartori, Roberto
dc.contributor.authorGuardieiro, Monique M.
dc.contributor.authorPerecin, Felipe
dc.contributor.authorMeirelles, Flvio V.
dc.contributor.authorSangalli, Juliano R.
dc.contributor.authorEberlin, Marcos N.
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionVitro Brasil Ltda
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2016-01-24T13:59:37Z
dc.date.available2016-01-24T13:59:37Z
dc.date.issued2010-05-01
dc.description.abstractMethods used for lipid analysis in embryos and oocytes usually involve selective lipid extraction from a pool of many samples followed by chemical manipulation, separation and characterization of individual components by chromatographic techniques. Herein we report direct analysis by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) of single and intact embryos or oocytes from various species. Biological samples were simply moisturized with the matrix solution and characteristic lipid ( represented by phosphatidylcholines, sphingomyelins and triacylglycerols) profiles were obtained via MALDI-MS. As representative examples, human, bovine, sheep and fish oocytes, as well as bovine and insect embryos were analyzed. MALDI-MS is shown to be capable of providing characteristic lipid profiles of gametes and embryos and also to respond to modifications due to developmental stages and in vitro culture conditions of bovine embryos. Investigation in developmental biology of the biological roles of structural and reserve lipids in embryos and oocytes should therefore benefit from these rapid MALDI-MS profiles from single and intact species.-Ferreira, C. R., S. A. Saraiva, R. R. Catharino, J. S. Garcia, F. C. Gozzo, G. B. Sanvido, L. F. A. Santos, E. G. Lo Turco, J. H. F. Pontes, A. C. Basso, R. P. Bertolla, R. Sartori, M. M. Guardieiro, F. Perecin, F. V. Meirelles, J. R. Sangalli, and M. N. Eberlin. Single embryo and oocyte lipid fingerprinting by mass spectrometry. J. Lipid Res. 2010. 51: 1218-1227.en
dc.description.affiliationUniv Estadual Campinas, Thomson Mass Spectrometry Lab, Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Dept Clin Pathol, Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Ctr Struct Mol Biol, Campinas, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Surg, São Paulo, Brazil
dc.description.affiliationVitro Brasil Ltda, Mogi Mirim, SP, Brazil
dc.description.affiliationUniv São Paulo, Dept Anim Sci, Piracicaba, SP, Brazil
dc.description.affiliationUniv São Paulo, Fac Anim Sci & Food Engn, Pirassununga, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Surg, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIDFAPESP: 2008/10756-7
dc.format.extent1218-1227
dc.identifierhttp://dx.doi.org/10.1194/jlr.D001768
dc.identifier.citationJournal of Lipid Research. Bethesda: Amer Soc Biochemistry Molecular Biology Inc, v. 51, n. 5, p. 1218-1227, 2010.
dc.identifier.doi10.1194/jlr.D001768
dc.identifier.issn0022-2275
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/32493
dc.identifier.wosWOS:000276633100038
dc.language.isoeng
dc.publisherAmer Soc Biochemistry Molecular Biology Inc
dc.relation.ispartofJournal of Lipid Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectoocyteen
dc.subjectembryoen
dc.subjectmass spectrometryen
dc.subjectphospholipiden
dc.subjecttriacylglicerolen
dc.subjectlipid fingerprintingen
dc.titleSingle embryo and oocyte lipid fingerprinting by mass spectrometryen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
Em verificação - Geral