Experimental Leishmania (L.) amazonensis leishmaniasis: Characterization and immunogenicity of subcellular fractions

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dc.contributor.authorValle, T. Zaverucha do
dc.contributor.authorGaspar, Emanuelle Baldo [UNIFESP]
dc.contributor.authorSouza-Lemos, C.
dc.contributor.authorSouza, C. S. F.
dc.contributor.authorMarquez, F. B. Zamora
dc.contributor.authorBaetas-da-Cruz, W.
dc.contributor.authord'Escofier, L. N.
dc.contributor.authorCorte-Real, S.
dc.contributor.authorCalabrese, K. S.
dc.contributor.authorCosta, S. C. Goncalves da
dc.contributor.institutionInst Oswaldo Cruz
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:41:45Z
dc.date.available2016-01-24T12:41:45Z
dc.date.issued2007-01-01
dc.description.abstractA technique developed in Trypanosoma cruzi biochemical studies was successfully used to fractionate Leishmania (Leishmania) amazonensis promastigotes. Ultrastructural analyses revealed a membrane fraction (MF) associated to subpellicular microtubules, a ribosomal-rich microsomal fraction (MicF), and a flagellar fraction (FF) free of associated membrane. All fractions proved to be immunogenic through delayed type hypersensitivity reaction assays. Therefore, a protocol was designed to test whether these fractions could elicit a protective response in mice infected by L. (L), amazonensis. the protocol consisted of a BCG injection (as cellular immunity inducer), followed by cyclophosphamide (once its cytotoxic effect is over, this immunosupressor can increase the number of circulating leukocytes), then an injection with one of the fractions followed by a challenge. When compared to infected control animals, mice injected with any of the fractions presented a smaller footpad swelling, especially those injected with MicF or FF. Macroscopically, immunized mice under modulation by BCG presented no swelling. Histopathological studies performed on day 120 revealed fewer amastigotes and more intense inflammation in lesions of MicF and FF injected mice. Animals injected with MF presented an intermediate pattern. Parasite quantification corroborated these results. the results show that all fractions are potent immunostimulators, but MicF and FF have the strongest protective ability.en
dc.description.affiliationInst Oswaldo Cruz, Dept Protozool, Lab Imunomodulacao, BR-21040900 Rio de Janeiro, Brazil
dc.description.affiliationInst Oswaldo Cruz, Dept Imunol, Lab Pesquisa Leishmanioses, BR-21040900 Rio de Janeiro, Brazil
dc.description.affiliationInst Oswaldo Cruz, Dept Ultraestrutura & Biol, Lab Ultraestrutura Celular, BR-21040900 Rio de Janeiro, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Disciplina Microbiol Imunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Disciplina Microbiol Imunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent473-492
dc.identifierhttp://dx.doi.org/10.1080/08820130701360972
dc.identifier.citationImmunological Investigations. Philadelphia: Taylor & Francis Inc, v. 36, n. 4, p. 473-492, 2007.
dc.identifier.doi10.1080/08820130701360972
dc.identifier.issn0882-0139
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/29400
dc.identifier.wosWOS:000248504800009
dc.language.isoeng
dc.publisherTaylor & Francis Inc
dc.relation.ispartofImmunological Investigations
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://journalauthors.tandf.co.uk/permissions/reusingOwnWork.asp
dc.subjectLeishmania (Leishmania) amazonesisen
dc.subjectsubcellular fractionationen
dc.subjectdelayed type hypersensitivityen
dc.subjectBCGen
dc.subjecttransmission electron microscopyen
dc.subjectmurine modelen
dc.titleExperimental Leishmania (L.) amazonensis leishmaniasis: Characterization and immunogenicity of subcellular fractionsen
dc.typeinfo:eu-repo/semantics/article
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