Special dyeing, histochemistry, immunohistochemistry and ultrastructure: A study of mast cells/eosinophilic granules cells (MCs/EGC) from Centropomus parallelus intestine

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dc.citation.volume60
dc.contributor.authorSilva, Wemeson Ferreira da [UNIFESP]
dc.contributor.authorSimões, Manuel de Jesus [UNIFESP]
dc.contributor.authorGutierre, Robson Campos [UNIFESP]
dc.contributor.authorEgami, Mizue Imoto [UNIFESP]
dc.contributor.authorSantos, Antenor Aguiar [UNIFESP]
dc.contributor.authorAntoniazzi, Marta M.
dc.contributor.authorSasso, Gisela Rodrigues da Silva [UNIFESP]
dc.contributor.authorRanzani-Paiva, Maria Jose T.
dc.coverageLondon
dc.date.accessioned2020-07-31T12:46:57Z
dc.date.available2020-07-31T12:46:57Z
dc.date.issued2017
dc.description.abstractIntestine mast cells/eosinophilic granule cells (MCs/EGC) of the marine species Centropomus parallelus (fat snook) were first studied using light and electron microscopy techniques. Mast cells are cells from the connective tissue found in almost all organs and tissues of vertebrates. In fish, they appear in greater numbers in parts of their bodies that are exposed to their environment, such as skin, gills and intestine. The granules in fat snook's mast cell contain a variety of substances, such as histamine, heparin, chondroitin sulfate, serotonin, proteases and cytokines. The present study of intestine MCs/EGC was carried out in 20 specimens of fat snook. Samples of tissue were fixed in Bouin solution and in buffered formalin. Ferric hematoxylin - Congo red, pH6 acridine orange, pH2.5 and pH0,5 Alcian Blue (AB), toluidine blue, PAS, AB + PAS and immunohistochemistry protocols were used. In the mucosa and submucosa layers, MCs/EGCs granules with basic contents were evidenced by Congo red staining, and with acid contents granules were identified through pH 2.5 and 0,5 AB, and acridine orange. Basic and acid contents were simultaneously evidenced using ferric hematoxylin - Congo red stain. Metachromasia was observed in both mucosal and submucosal mast cells. Neutral glycoproteins were evidenced by using PAS protocol, glycosaminoglycan through AB and both simultaneously through AB + PAS. In immunohistochemistry assays, MCs/EGC were positive for tryptase, chymase and serotonin. As in mammals, the study of samples fixed in modified Karnovsky for transmission electron microscopy evidenced that most of the MCs granules were spherical and showed varying electron density, as described in previous reports on other teleost fish species. The metachromasia observed and the identification of tryptase, chymase and serotonin suggest a great similarity between fat snook's MCs/EGC and those described in the mucosa of mammals. (C) 2016 Elsevier Ltd. All rights reserved.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Morphol & Genet, Lab Histol & Struct Biol, Sao Paulo, SP, Brazil
dc.description.affiliationAdventist Univ Ctr Sao Paulo, Dept Morphol, Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Neurol & Neurosurg, Lab Neurophysiol & Exercise Physiol, Sao Paulo, SP, Brazil
dc.description.affiliationButantan Inst, Cell Biol Lab, Sao Paulo, SP, Brazil
dc.description.affiliationFishery Inst, Sao Paulo, SP, Brazil
dc.description.affiliationUnifespDepartment of Morphology and Genetics, Laboratory of Histology and Structural Biology, Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil
dc.description.affiliationUnifespDepartment of Neurology and Neurosurgery, Laboratory of Neurophysiology and Exercise Physiology, Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipNational Council for Scientific and Technological Development (CNPq) [559760/2009-4]
dc.description.sponsorshipCoordination for the Improvement of Higher Education Personnel foundation (CAPES, CAPES PNPD) scholarship
dc.format.extent502-508
dc.identifierhttp://dx.doi.org/10.1016/j.fsi.2016.11.022
dc.identifier.citationFish & Shellfish Immunology. London, v. 60, p. 502-508, 2017.
dc.identifier.doi10.1016/j.fsi.2016.11.022
dc.identifier.issn1050-4648
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56472
dc.identifier.wosWOS:000392770000055
dc.language.isoeng
dc.publisherAcademic Press Ltd- Elsevier Science Ltd
dc.relation.ispartofFish & Shellfish Immunology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectHistochemistryen
dc.subjectImmunohistochemistryen
dc.subjectUltrastructureen
dc.subjectMast cellsen
dc.subjectCentropomus parallelusen
dc.titleSpecial dyeing, histochemistry, immunohistochemistry and ultrastructure: A study of mast cells/eosinophilic granules cells (MCs/EGC) from Centropomus parallelus intestineen
dc.typeinfo:eu-repo/semantics/article
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