Lutein and Zeaxanthin Toxicity With and Without Brilliant Blue in Rabbits
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2014-09-01
Autores
Furlani, Bruno Albuquerque 
Barroso, Leticia
Sousa-Martins, Diogo
Maia, Mauricio
Moraes-Filho, Milton Nunes
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Purpose: To evaluate the safety profile of solutions containing lutein and zeaxanthin alone or associated with brilliant blue (BB).Methods: Twenty-eight New Zealand rabbits were used to evaluate 4 concentrations of the various dye solutions: 0.5% lutein/zeaxanthin; 0.5% lutein/zeaxanthin associated with 0.0125% BB; 0.3% lutein/zeaxanthin associated with 0.025% BB; and 0.25% lutein/zeaxanthin associated with 0.05% BB. the pHs of the dye solutions ranged from 6.5 to 7.2 and the osmolarities from 280 to 320 mOsm/mL. Each rabbit had 0.1mL of one of the dyeing solutions injected into the vitreous cavity of the right eye, while balanced salt solution (BSS) was injected into the left eye as the control. Scotopic electroretinography responses were recorded in all eyes at different time points. the animals were sacrificed at 1 and 7 days after injection; the eyes were analyzed by light and transmission electron microscopy.Results: No significant (P > 0.05) differences were seen in the a- and b-wave amplitudes among groups at any given point in time. Light and electron microscopy findings showed no significant abnormalities either, and were similar to the histological findings after intravitreal BSS injection.Conclusions: Lutein and zeaxanthin alone or in association with BB showed a good safety profile in this experimental model.
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Journal of Ocular Pharmacology and Therapeutics. New Rochelle: Mary Ann Liebert, Inc, v. 30, n. 7, p. 559-566, 2014.