The effect of thyroid hormones on the white adipose tissue gene expression of PAI-1 and its serum concentration

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dc.contributor.authorBiz, Carolina [UNIFESP]
dc.contributor.authorOliveira, Cristiane de [UNIFESP]
dc.contributor.authorMattos, Ana Barbosa Marcondes de [UNIFESP]
dc.contributor.authorOliveira, Juliana de. [UNIFESP]
dc.contributor.authorRibeiro, Eliane Beraldi [UNIFESP]
dc.contributor.authorNascimento, Claudia Maria da Penha Oller do [UNIFESP]
dc.contributor.authorOyama, Lila Missae [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-06-14T13:41:16Z
dc.date.available2015-06-14T13:41:16Z
dc.date.issued2009-12-01
dc.description.abstractMetabolic syndrome is associated with an increased risk of developing cardiovascular diseases and Plasminogen activator inhibitor 1 (PAI-1) overexpression may play a significant role in this process. A positive correlation between adipose tissue gene expression of PAI-1 and its serum concentration has been reported. Furthermore, high serum levels of thyroid hormones (T3 and T4) and PAI-1 have been observed in obese children. The present study evaluates the impact of thyroid hormone treatment on white adipose tissue PAI-1 gene expression and its serum concentration. Male Wistar rats (60 days old) were treated for three weeks with T4 (50 µg/day, Hyper) or with saline (control). Additionally, 3T3-L1 adipocytes were treated for 24 h with T4 (100 nM) or T3 (100 nM). PAI-1 gene expression was determined by real-time PCR, while the serum concentration of PAI-1 was measured by ELISA using a commercial kit (Innovative Research, USA). Both the serum concentration of PAI-1 and mRNA levels were similar between groups in retroperitoneal and epididymal white adipose tissue. Using 3T3-L1 adipocytes, in vitro treatment with T4 and T3 increased the gene expression of PAI-1, suggesting non-genomic and genomic effects, respectively. These results demonstrate that thyroid hormones have different effects in vitro and in vivo on PAI-1 gene expression in adipocytes.en
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Departamento de Fisiologia Disciplina de Fisiologia da Nutrição
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Departamento de Biociências
dc.description.affiliationUnifespUNIFESP, Depto. de Fisiologia Disciplina de Fisiologia da Nutrição
dc.description.affiliationUnifespUNIFESP, Depto. de Biociências
dc.description.sourceSciELO
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 2006/58628-1
dc.format.extent1163-1166
dc.identifierhttp://dx.doi.org/10.1590/S0100-879X2009007500013
dc.identifier.citationBrazilian Journal of Medical and Biological Research. Associação Brasileira de Divulgação Científica, v. 42, n. 12, p. 1163-1166, 2009.
dc.identifier.doi10.1590/S0100-879X2009007500013
dc.identifier.fileS0100-879X2009001200007.pdf
dc.identifier.issn0100-879X
dc.identifier.scieloS0100-879X2009001200007
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/5344
dc.identifier.wosWOS:000272487500007
dc.language.isoeng
dc.publisherAssociação Brasileira de Divulgação Científica
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectThyroid hormonesen
dc.subjectPlasminogen activator inhibitor 1en
dc.subjectWhite adipose tissueen
dc.subject3T3-L1en
dc.titleThe effect of thyroid hormones on the white adipose tissue gene expression of PAI-1 and its serum concentrationen
dc.typeinfo:eu-repo/semantics/article
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