Avaliação dos efeitos da fotobiomodulação e da suplementação com ômega-3 em modelo animal de acidente vascular cerebral isquêmico
Arquivos
Data
2021
Autores
Vogel, Debora Daisy da Silva 
Orientadores
Scorza, Carla Alessandra
Tipo
Tese de doutorado
Título da Revista
ISSN da Revista
Título de Volume
Resumo
Objetivo: O AVC tem uma alta carga de morbimortalidade e é um enorme problema de saúde pública em todo o mundo. O tratamento para o AVC isquêmico agudo é subutilizado, atingindo apenas uma pequena porcentagem dos pacientes. Por esse motivo, novas abordagens no tratamento de AVC são necessárias. A fotobiomodulação e a suplementação com ômega-3 são abordagens terapêuticas potenciais após o AVC, com o objetivo de restaurar a região peri-lesional, ainda viável e com potencial de recuperação. Este estudo avaliou os efeitos da fotobiomodulação e da suplementação de ômega-3 na dinâmica cerebral após acidente vascular cerebral isquêmico focal em ratos. Métodos: O AVC isquêmico foi induzido por fototrombose. Foram utilizados 66 ratos Wistar machos (230 ± 20g), distribuídos aleatoriamente em 5 grupos experimentais, a saber: Grupo Sham (sem AVC), Grupo Controle de AVC (AVCC), Grupo AVC Omega (AVCO), Stroke Group PBM / Laser Therapy (AVCL), Stroke Omega e Laser Therapy Group (AVCOL). Os tratamentos consistiram em: (1) 85mg / kg / dia de óleo de peixe (CATARINENSE®) via gavagem oral por 60 dias consecutivos após a indução do AVC, e (2) fotobiomodulação aplicada por meio de equipamento de diodo laser (MM Optics), com comprimento de onda de 780 nm, 3x / semana por 2 meses. Após o término dos tratamentos foi utilizada estereologia para avaliação do volume da lesão isquêmica, análise imunoistoquímica foi utilizada para revelar a expressão de NeuN, Iba e GFAP para investigar células neuronais e gliais enquanto citocinas inflamatórias (TNF-α, IL-1β, IL -6, IL-10 e TGF-β) e neurotrofinas (VEGF, BDNF, TrkB e ANGIO II) foram detectadas por meio de teste imunoenzimático. Além disso, sessenta dias após o término dos tratamentos, a atividade elétrica cortical e talâmica foi registrada por eletroencefalografia. Resultados e conclusões: (1) Os animais tratados com ômega-3 apresentaram atividade microglial reduzida. (2) A associação de ômega-3 e fotobiomodulação foi eficaz na redução da atividade microglial e na redução da duração e do número de crises eletrográficas. Porém, (3) o uso da fotobiomodulação isolada foi mais eficaz, e promoveu redução do volume da lesão isquêmica, redução das citocinas pró-inflamatórias, aumento astroglial e redução da microglia na região peri-lesional, redução na duração e no número de convulsões eletrográficas em animais com epilepsia adquirida pós-lesão. Nossos resultados sugerem efeitos neuroprotetores da fotobiomodulação e seu potencial terapêutico como ferramenta auxiliar no tratamento pós-AVC.
Objective: Stroke has a high burden of morbidity and mortality and is an enormous public health problem worldwide The treatment for acute ischemic stroke is underused, reaching only a small percentage of patients. For this reason, new approaches in AVC treatment are necessary. Photobiomodulation and omega-3 supplementation are potential therapeutic approaches after stroke, aimed to restore the peri-lesional region, which is still viable and with potential for recovery. This study evaluated the effects of photobiomodulation and omega-3 supplementation on brain dynamics after focal ischemic stroke in rats. Methods: Ischemic stroke was induced by photothrombosis. We used 66 male Wistar rats (230 ± 20g), randomly distributed into 5 experimental groups, namely: Sham Group (without stroke), Stroke Control Group (AVCC), Stroke Omega Group (AVCO), Stroke Group PBM/ Laser Therapy (AVCL), Stroke Omega and Laser Therapy Group (AVCOL). The treatments consisted of: (1) 85mg/kg/day of fish oil (CATARINENSE®) via oral gavage for 60 consecutive days after stroke induction, and (2) photobiomodulation applied through a diode laser equipment (MM Optics), with a wavelength of 780nm, 3x/week for 2 months. After the end of treatments stereology was used to evaluate the volume of the ischemic lesion, immunohistochemical analysis was used to reveal NeuN, Iba and GFAP expression in order to investigate neuronal and glial cells while inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-10 and TGF-β) and neurotrophins (VEGF, BDNF, TrkB and ANGIO II) were detected by means of an immunoenzymatic test. Furthermore, sixty days after the end of treatments, cortical and thalamic electrical activity were recorded by electroencephalography. Results and Conclusions: (1) Animals treated with omega-3 showed reduced microglial activity. (2) The association of omega-3 and photobiomodulation was effective in reducing microglial activity and in reducing the duration and number of electrographic seizures. However, (3) the use of photobiomodulation alone was more effective, and promoted reduction of the ischemic lesion volume, reduction of pro-inflammatory cytokines, astroglial enlargement and microglial reduction in the peri-lesional region, reduction in duration and in the number of electrographic seizures in animals with acquired post-injury epilepsy. Our results suggest neuroprotective effects of photobiomodulation and its therapeutic potential as an auxiliary tool in post-stroke treatment.
Objective: Stroke has a high burden of morbidity and mortality and is an enormous public health problem worldwide The treatment for acute ischemic stroke is underused, reaching only a small percentage of patients. For this reason, new approaches in AVC treatment are necessary. Photobiomodulation and omega-3 supplementation are potential therapeutic approaches after stroke, aimed to restore the peri-lesional region, which is still viable and with potential for recovery. This study evaluated the effects of photobiomodulation and omega-3 supplementation on brain dynamics after focal ischemic stroke in rats. Methods: Ischemic stroke was induced by photothrombosis. We used 66 male Wistar rats (230 ± 20g), randomly distributed into 5 experimental groups, namely: Sham Group (without stroke), Stroke Control Group (AVCC), Stroke Omega Group (AVCO), Stroke Group PBM/ Laser Therapy (AVCL), Stroke Omega and Laser Therapy Group (AVCOL). The treatments consisted of: (1) 85mg/kg/day of fish oil (CATARINENSE®) via oral gavage for 60 consecutive days after stroke induction, and (2) photobiomodulation applied through a diode laser equipment (MM Optics), with a wavelength of 780nm, 3x/week for 2 months. After the end of treatments stereology was used to evaluate the volume of the ischemic lesion, immunohistochemical analysis was used to reveal NeuN, Iba and GFAP expression in order to investigate neuronal and glial cells while inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-10 and TGF-β) and neurotrophins (VEGF, BDNF, TrkB and ANGIO II) were detected by means of an immunoenzymatic test. Furthermore, sixty days after the end of treatments, cortical and thalamic electrical activity were recorded by electroencephalography. Results and Conclusions: (1) Animals treated with omega-3 showed reduced microglial activity. (2) The association of omega-3 and photobiomodulation was effective in reducing microglial activity and in reducing the duration and number of electrographic seizures. However, (3) the use of photobiomodulation alone was more effective, and promoted reduction of the ischemic lesion volume, reduction of pro-inflammatory cytokines, astroglial enlargement and microglial reduction in the peri-lesional region, reduction in duration and in the number of electrographic seizures in animals with acquired post-injury epilepsy. Our results suggest neuroprotective effects of photobiomodulation and its therapeutic potential as an auxiliary tool in post-stroke treatment.
Descrição
Citação
VOGEL, D. D.S. Avaliação dos efeitos da fotobiomodulação e da suplementação com ômega-3 em modelo animal de acidente vascular cerebral isquêmico. São Paulo, 2021. 79 f. Tese (Doutorado em Neurologia/Neurociências) - Escola Paulista de Medicina), Universidade Federal de São Paulo (UNIFESP), São Paulo, 2021.