New Trypanosoma cruzi repeated element that shows site specificity for insertion

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dc.contributor.authorSouza, Renata Torres [UNIFESP]
dc.contributor.authorSantos, Marcia Regina Machado [UNIFESP]
dc.contributor.authorLima, Fabio Mitsuo [UNIFESP]
dc.contributor.authorEl-Sayed, Najib M.
dc.contributor.authorMyler, Peter J.
dc.contributor.authorRuiz, Jeronimo Conceição
dc.contributor.authorSilveira, Jose Franco da [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionSeattle Biomed Res Inst
dc.contributor.institutionInst Genom Res
dc.contributor.institutionFiocruz MS
dc.date.accessioned2016-01-24T13:48:49Z
dc.date.available2016-01-24T13:48:49Z
dc.date.issued2007-07-01
dc.description.abstractA new family of site-specific repeated elements identified in Trypanosoma cruzi, which we named TcTREZO, is described here. TcTREZO appears to be a composite repeated element, since three subregions may be defined within it on the basis of sequence similarities with other T. cruzi sequences. Analysis of the distribution of TcTREZO in the genome clearly indicates that it displays site specificity for insertion. Most TcTREZO elements are flanked by conserved sequences. There is a highly conserved 68-bp sequence at the 5' end of the element and a sequence domain of similar to 500 bp without a well-defined borderline at the 3' end. Northern blot hybridization and reverse transcriptase PCR analyses showed that TcTREZO transcripts are expressed as oligo(A) -terminated transcripts whose length corresponds to the unit size of the element (1.6 kb). Transcripts of similar to 0.2 kb derived from a small part of TcTREZO are also detected in steady-state RNA. TcTREZO transcripts are unspliced and not translated. the copy number of TcTREZO sequences was estimated to be similar to 173 copies per haploid genome. TcTREZO appears to have been assembled by insertions of sequences into a progenitor element. Once associated with each other, these subunits were amplified as a new transposable element. TcTREZO shows site specificity for insertion, suggesting that a sequence-specific endonuclease could be responsible for its insertion at a unique site.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationSeattle Biomed Res Inst, Seattle, WA 98109 USA
dc.description.affiliationInst Genom Res, Rockville, MD USA
dc.description.affiliationFiocruz MS, Rene Rachou Res Ctr, CPqRR, Belo Horizonte, MG, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)pt
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)pt
dc.format.extent1228-1238
dc.identifierhttps://dx.doi.org/10.1128/EC.00036-07
dc.identifier.citationEukaryotic Cell. Washington: Amer Soc Microbiology, v. 6, n. 7, p. 1228-1238, 2007.
dc.identifier.doi10.1128/EC.00036-07
dc.identifier.fileWOS000248133100016.pdf
dc.identifier.issn1535-9778
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/29832
dc.identifier.wosWOS:000248133100016
dc.language.isoeng
dc.publisherAmer Soc Microbiology
dc.relation.ispartofEukaryotic Cell
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleNew Trypanosoma cruzi repeated element that shows site specificity for insertionen
dc.typeinfo:eu-repo/semantics/article
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