Distribution of Trypanosoma cruzi stage-specific epitopes in cardiac muscle of Calomys callosus, BALB/c mice, and cultured cells infected with different infective forms

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dc.contributor.authorTaniwaki, Noerni N.
dc.contributor.authorSilva, Claudio Vieira da
dc.contributor.authorSilva, Solange da
dc.contributor.authorMortara, Renato A.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionSecao Microscopia Eletron Inst Adolfo Lutz
dc.date.accessioned2016-01-24T13:48:50Z
dc.date.available2016-01-24T13:48:50Z
dc.date.issued2007-07-01
dc.description.abstractTo examine whether distinct parasite infective forms or the mammalian host could affect the distribution of Trypanosoma cruzi stage-specific epitopes defined by monoclonal antibodies (Mabs) raised against mammalian-stage parasite forms, immunofluorescence studies followed the intracellular life cycle of the parasite in the cardiac muscle of Calomys callosus and BALB/c mice in the acute phase of the disease and in LLC-MK(2) Cultured cells. Animals and cells were infected either with tissue-culture derived trypomastigotes (TCT) or bloodstream trypornastigotes (BT) from the Y strain of T cruzi. Samples were examined under confocal fluorescence microscopy after labeling with Mabs 2C2, 1D9, 2B7, 3G8, 3B9, and 4B9 that react with carbohydrate epitopes on Ssp-4, a major amastigote surface glycoprotein; Mab 4B5 that identifies a noncarbohydrate epitope on all intracellular parasites stages, and Mab 3B2 that also recognizes a noncarbohydrate epitope expressed only in flagellated forms. Samples were double labeled with DAPI to visualize parasites' kinetoplasts and nuclei. Most of the Mabs used in this work displayed a surface labeling pattern on amastigotes present in Calomys and mice hearts, and in LLC-MK2 cultured cells infected with BT or TCT. Mab 2B7, however, displayed a marked polymorphic distribution in antigen expression between both mammalian hosts, independent on the infective form. Beyond the polymorphic distribution of amastigote surface epitopes, Calomys, and mice heart sections presented several inflammatory cells around amastigotes and trypornastigotes nests. (c) 2007 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, Disciplina Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationSecao Microscopia Eletron Inst Adolfo Lutz, BR-01246902 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, Disciplina Parasitol, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent14-25
dc.identifierhttp://dx.doi.org/10.1016/j.actatropica.2007.05.007
dc.identifier.citationActa Tropica. Amsterdam: Elsevier B.V., v. 103, n. 1, p. 14-25, 2007.
dc.identifier.doi10.1016/j.actatropica.2007.05.007
dc.identifier.issn0001-706X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/29848
dc.identifier.wosWOS:000248929700002
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofActa Tropica
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjecttrypanosoma cruzien
dc.subjectamastigoteen
dc.subjectarypomastigoteen
dc.subjectmonoclonal antibodyen
dc.subjectepitopeen
dc.subjectConfocal microscopyen
dc.titleDistribution of Trypanosoma cruzi stage-specific epitopes in cardiac muscle of Calomys callosus, BALB/c mice, and cultured cells infected with different infective formsen
dc.typeinfo:eu-repo/semantics/article
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