The Repetitive Cytoskeletal Protein H49 of Trypanosoma cruzi Is a Calpain-Like Protein Located at the Flagellum Attachment Zone

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dc.contributor.authorGaletovic, Alexandra [UNIFESP]
dc.contributor.authorSouza, Renata Torres [UNIFESP]
dc.contributor.authorSantos, Marcia Regina Machado
dc.contributor.authorCordero, Esteban Mauricio [UNIFESP]
dc.contributor.authorBastos, Izabela Marques Dourado
dc.contributor.authorSantana, Jaime Martins de
dc.contributor.authorRuiz, Jeronimo Conceição
dc.contributor.authorLima, Fabio Mitsuo [UNIFESP]
dc.contributor.authorMarini, Marjorie Mendes [UNIFESP]
dc.contributor.authorMortara, Renato Arruda [UNIFESP]
dc.contributor.authorSilveira, Jose Franco da [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Antofagasta
dc.contributor.institutionUniv Bandeirante São Paulo
dc.contributor.institutionUniversidade de Brasília (UnB)
dc.contributor.institutionFiocruz MS
dc.date.accessioned2016-01-24T14:17:26Z
dc.date.available2016-01-24T14:17:26Z
dc.date.issued2011-11-11
dc.description.abstractBackground: Trypanosoma cruzi has a single flagellum attached to the cell body by a network of specialized cytoskeletal and membranous connections called the flagellum attachment zone. Previously, we isolated a DNA fragment (clone H49) which encodes tandemly arranged repeats of 68 amino acids associated with a high molecular weight cytoskeletal protein. in the current study, the genomic complexity of H49 and its relationships to the T. cruzi calpain-like cysteine peptidase family, comprising active calpains and calpain-like proteins, is addressed. Immunofluorescence analysis and biochemical fractionation were used to demonstrate the cellular location of H49 proteins.Methods and Findings: All of H49 repeats are associated with calpain-like sequences. Sequence analysis demonstrated that this protein, now termed H49/calpain, consists of an amino-terminal catalytic cysteine protease domain II, followed by a large region of 68-amino acid repeats tandemly arranged and a carboxy-terminal segment carrying the protease domains II and III. the H49/calpains can be classified as calpain-like proteins as the cysteine protease catalytic triad has been partially conserved in these proteins. the H49/calpains repeats share less than 60% identity with other calpain-like proteins in Leishmania and T. brucei, and there is no immunological cross reaction among them. It is suggested that the expansion of H49/calpain repeats only occurred in T. cruzi after separation of a T. cruzi ancestor from other trypanosomatid lineages. Immunofluorescence and immunoblotting experiments demonstrated that H49/calpain is located along the flagellum attachment zone adjacent to the cell body.Conclusions: H49/calpain contains large central region composed of 68-amino acid repeats tandemly arranged. They can be classified as calpain-like proteins as the cysteine protease catalytic triad is partially conserved in these proteins. H49/calpains could have a structural role, namely that of ensuring that the cell body remains attached to the flagellum by connecting the subpellicular microtubule array to it.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, Escola Paulista Med, São Paulo, Brazil
dc.description.affiliationUniv Antofagasta, Lab Bioquim, Dept Biomed, Antofagasta, Chile
dc.description.affiliationUniv Bandeirante São Paulo, São Paulo, Brazil
dc.description.affiliationUniv Brasilia, Dept Biol Celular, Inst Biol, Brasilia, DF, Brazil
dc.description.affiliationFiocruz MS, Ctr Pesquisa Rene Rachou CPqRR, Belo Horizonte, MG, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, Escola Paulista Med, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipBeca Presidente de la Republica-Chile
dc.format.extent12
dc.identifierhttps://dx.doi.org/10.1371/journal.pone.0027634
dc.identifier.citationPlos One. San Francisco: Public Library Science, v. 6, n. 11, 12 p., 2011.
dc.identifier.doi10.1371/journal.pone.0027634
dc.identifier.fileWOS000297553900065.pdf
dc.identifier.issn1932-6203
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/34223
dc.identifier.wosWOS:000297553900065
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPlos One
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleThe Repetitive Cytoskeletal Protein H49 of Trypanosoma cruzi Is a Calpain-Like Protein Located at the Flagellum Attachment Zoneen
dc.typeinfo:eu-repo/semantics/article
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