Histological changes underlying bupivacaine's effect on extra ocular muscle

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dc.contributor.authorHopker, Luisa Moreira [UNIFESP]
dc.contributor.authorNeves, Juliana de Carvalho
dc.contributor.authorNascimento, Daiane Jaqueline
dc.contributor.authorCampos, Eliene Dutra
dc.contributor.authorMendonca, Tomas Scalamandre [UNIFESP]
dc.contributor.authorZanoteli, Edmar
dc.contributor.authorAllemann, Norma [UNIFESP]
dc.date.accessioned2018-07-26T12:18:33Z
dc.date.available2018-07-26T12:18:33Z
dc.date.issued2018
dc.description.abstractTo determine the changes in the cross-sectional area (CSA) of myofibers and their subtype distribution based on the myosin isoform expression after bupivacaine (BUP) injection in the EOM of rabbits and help the understanding of strabismus correction after BUP injection in the clinical practice. A total of 32 rabbits received 0.3 mL of 1.5% BUP in the superior rectus muscle (SR) of the right eye (OD) and were sacrificed at days 7, 28, 60, and 92. Additional eight untouched rabbits were included as controls. Hematoxylin and eosin staining was performed, and ImageJ software was used to measure CSA. Immunohistochemical analysis was performed to analyze the proportion of myofibers positive for myosin types 1 (slow), 2 (fast) and embryonic. Myofiber area measurement decreased 7 days after BUP injection [SR, 1271 +/- 412 mu m(2) (control) to 909 +/- 255 mu m(2) (day 7)] after BUP injection, followed by an increasing trend after 28 days and normalization after 92 days [SRen
dc.description.abstract1062 +/- 363 mu m(2) (day 28), 1492 +/- 404 mu m(2) (day 60), 1317 +/- 334 mu m(2) (day 92)]. The proportion of slow myosin-positive fibers increased in the 60-day group (88.5% +/- 16.2%). There was no statistically significant difference in fast myosin-positive fibers. The inferior rectus of both eyes showed an increase in CSA. No increase of endomysial fibrous tissue was observed after 60 and 92 days of BUP injection. Bupivacaine, when injected into the SR of rabbits, initially decreases the fiber area followed by a transient increasing trend and normalization. There is a transient increase in the proportion of slow myosin-positive fibers in the injected muscle. Muscle adaptation in untreated EOM was found with increased CSA. These findings help clarify the clinical effects of BUP in extraocular muscle.en
dc.description.affiliationFed Univ Sao Paulo UNIFESP, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Fac Med, Sao Paulo, Brazil
dc.description.affiliationEvangel Hosp Curitiba, Curitiba, Parana, Brazil
dc.description.affiliationUIC, Chicago, IL USA
dc.description.affiliationUnifespFed Univ Sao Paulo UNIFESP, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCNPq
dc.description.sponsorshipDepartment of Ophthalmology at the Federal University of Sao Paulo
dc.description.sponsorshipCAPES Doctorate scholarship
dc.format.extent62-67
dc.identifierhttp://dx.doi.org/10.1016/j.exer.2018.03.004
dc.identifier.citationExperimental Eye Research. London, v. 171, p. 62-67, 2018.
dc.identifier.doi10.1016/j.exer.2018.03.004
dc.identifier.issn0014-4835
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/45980
dc.identifier.wosWOS:000433646000007
dc.language.isoeng
dc.publisherAcademic Press Ltd- Elsevier Science Ltd
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectExtraocular muscleen
dc.subjectBupivacaineen
dc.subjectStrabismusen
dc.subjectMuscle physiologyen
dc.subjectRegenerationen
dc.subjectSlow myosinen
dc.titleHistological changes underlying bupivacaine's effect on extra ocular muscleen
dc.typeinfo:eu-repo/semantics/article
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