Spectrofluorimetric Determination of Coumarin in Commercial Tablets

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dc.contributor.authorMarcolan, Mirian
dc.contributor.authorMartins, Priscila Alfonso
dc.contributor.authorPedrosa, Valber A.
dc.contributor.authorRodrigues, Maira R.
dc.contributor.authorOliveira, Hueder P. M. de
dc.contributor.authorCodognoto, Lucia [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Vale do Paraiba
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal Fluminense (UFF)
dc.contributor.institutionUniv Camilo Castelo Branco
dc.date.accessioned2016-01-24T14:06:15Z
dc.date.available2016-01-24T14:06:15Z
dc.date.issued2011-03-01
dc.description.abstractA simple, rapid and effective analytical method based on fluorescence spectroscopy for the determination of coumarin in pharmaceutical formulations without pre-treatment or pre-concentration step was development. Coumarin had maximum excitation and emission at 310 nm and 390 nm, respectively. Optimum conditions for the detection of coumarin were investigated. Under optimized conditions, we observed a linear behavior for the sign of coumarin in the concentration range of 2.5 x 10(-6) to 1.0 x 10(-4) mol L(-1), with linearity of 0.998 and sensitivity of 2.9 x 10(10) u.a/mol L(-1). the proposed method was validated in terms of accuracy, precision and specificity of coumarin using the standard addition and external calibration. It was noted that the results support (P < 0.05), indicating that the matrices were not an interference in the determination of coumarin by fluorescence spectroscopy. the results were favorable compared with those obtained by reference chromatographic methods.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Ciencias Exatas & Terra, BR-09972270 Diadema, SP, Brazil
dc.description.affiliationUniv Vale do Paraiba, Inst Pesquisa & Desenvolvimento, Sao Jose Dos Campos, SP, Brazil
dc.description.affiliationUNESP, Inst Biosci, Dept Chem & Biochem, Botucatu, SP, Brazil
dc.description.affiliationPolo Univ Rio das Ostras, Univ Fed Fluminense, Dept Ciencia & Tecnol, Rio Das Ostras, RJ, Brazil
dc.description.affiliationUniv Camilo Castelo Branco, BR-12247004 Sao Jose Dos Campos, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Ciencias Exatas & Terra, BR-09972270 Diadema, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipL 'Oreal/Academia Brasileira de Ciencias/Unesco
dc.description.sponsorshipIDCNPq: 479655/2008-1
dc.description.sponsorshipIDFAPESP: 08/50588-6
dc.description.sponsorshipIDFAPESP: 06/56701-3
dc.format.extent733-738
dc.identifierhttp://dx.doi.org/10.1007/s10895-010-0763-5
dc.identifier.citationJournal of Fluorescence. New York: Springer/plenum Publishers, v. 21, n. 2, p. 733-738, 2011.
dc.identifier.doi10.1007/s10895-010-0763-5
dc.identifier.issn1053-0509
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/33511
dc.identifier.wosWOS:000291061500029
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofJournal of Fluorescence
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.subjectCoumarinen
dc.subjectFluorescence spectroscopyen
dc.subjectPharmaceutical preparationsen
dc.titleSpectrofluorimetric Determination of Coumarin in Commercial Tabletsen
dc.typeinfo:eu-repo/semantics/article
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