Avaliação do sistema serotoninérgico no hipocampo de ratos obesos submetidos à troca de dieta e/ou treinamento físico
Data
2024-11-18
Autores
Cezar, Maria Eduarda Rodrigues 
Orientadores
Silva, Cristiano Mendes da
Tipo
Trabalho de conclusão de curso
Título da Revista
ISSN da Revista
Título de Volume
Resumo
Introdução: A obesidade vem sendo associada ao desenvolvimento de doenças crônicas e problemas no sistema serotoninérgico, tornando necessária a exploração de intervenções como a troca de dieta e treinamento físico. Objetivo: Avaliar o efeito do treinamento físico e da intervenção dietética no sistema serotoninérgico do hipocampo de ratos obesos. Métodos: Utilizou-se 30 ratos Wistar machos, com 8 semanas de vida. Com exceção do grupo controle (CC), os demais animais receberam uma dieta hiperlipídica palatável (DHL) por 20 semanas. Em seguida os ratos obesos foram distribuídos em 4 grupos: Sedentário Hiperlipídica (SHM), Sedentário Dieta (SDM), Treinado Hiperlipídica (THM) e Treinado Dieta (TDM). Os grupos SHM e THM permaneceram recebendo a DHL, enquanto os grupos SDM e TDM passaram a receber dieta padrão e os grupos THM e TDM realizaram um protocolo de exercício combinado. Após a intervenção, foi realizada eutanásia dos animais para avaliação da expressão proteica dos receptores de serotonina 5HT1A e 5HT2C no hipocampo. Resultados: A DHL foi efetiva na indução de obesidade no modelo escolhido. Na etapa de intervenção, a mudança para uma dieta controle foi a principal responsável pela redução da adiposidade visceral, com colaboração do treinamento na redução da gordura gonadal, especificamente. Conclusão: O protocolo de indução à obesidade via dieta se mostrou efetivo, com intervenções de treinamento e dieta agindo de maneira eficaz na redução da adiposidade visceral. Porém, o protocolo de treinamento combinado não afetou as moléculas do sistema serotoninérgico analisadas.
Introduction: Obesity has been associated with the development of chronic diseases and problems in the serotonergic system, making it necessary to explore interventions like changing diet and physical training Objective: To evaluate the effect of physical training and dietary intervention on the serotonergic system of the hippocampus of obese rats. Methods: Thirty male Wistar rats, 8 weeks old, were used. With the exception of the control group (CC), the other animals received a palatable high-fat diet (HFD) for 20 weeks. Then, the obese rats were distributed into 4 groups: Sedentary High-Fat (SHM), Sedentary Standard (SSM), Trained High-Fat (THM) and Trained Standard (TSM). The SHM and THM groups continued to receive HFD, while the SSM and TSM groups began to receive a standard diet while the THM and TDM groups underwent a combined exercise protocol. After the intervention, the animals were euthanized to evaluate the protein expression of serotonin 5HT1A and 5HT2C receptors in the hippocampus. Results: HFD was effective in inducing obesity in the chosen model. In the intervention stage, the change to a control diet was the main factor responsible for the reduction in visceral adiposity, with training collaborating in the reduction of gonadal fat, specifically. Conclusion: The obesity induction protocol via diet was effective, with training and diet interventions acting effectively in reducing visceral adiposity. However, the protocol did not affect the molecules of the serotonergic system.
Introduction: Obesity has been associated with the development of chronic diseases and problems in the serotonergic system, making it necessary to explore interventions like changing diet and physical training Objective: To evaluate the effect of physical training and dietary intervention on the serotonergic system of the hippocampus of obese rats. Methods: Thirty male Wistar rats, 8 weeks old, were used. With the exception of the control group (CC), the other animals received a palatable high-fat diet (HFD) for 20 weeks. Then, the obese rats were distributed into 4 groups: Sedentary High-Fat (SHM), Sedentary Standard (SSM), Trained High-Fat (THM) and Trained Standard (TSM). The SHM and THM groups continued to receive HFD, while the SSM and TSM groups began to receive a standard diet while the THM and TDM groups underwent a combined exercise protocol. After the intervention, the animals were euthanized to evaluate the protein expression of serotonin 5HT1A and 5HT2C receptors in the hippocampus. Results: HFD was effective in inducing obesity in the chosen model. In the intervention stage, the change to a control diet was the main factor responsible for the reduction in visceral adiposity, with training collaborating in the reduction of gonadal fat, specifically. Conclusion: The obesity induction protocol via diet was effective, with training and diet interventions acting effectively in reducing visceral adiposity. However, the protocol did not affect the molecules of the serotonergic system.
Descrição
Citação
CEZAR, Maria Eduarda Rodrigues. Avaliação do sistema serotoninérgico no hipocampo de ratos obesos submetidos à troca de dieta e/ou treinamento físico. 2024. 22 f. Trabalho de conclusão de curso (Graduação em Nutrição) - Universidade Federal de São Paulo, Instituto de Saúde e Sociedade, Santos, 2024.