The role of HiTI, a serine protease inhibitor from Haematobia irritans irritans (Diptera : Muscidae) in the control of fly and bacterial proteases

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dc.contributor.authorAzzolini, S. S.
dc.contributor.authorSasaki, S. D.
dc.contributor.authorCampos, ITN
dc.contributor.authorTorquato, RJS
dc.contributor.authorJuliano, M. A.
dc.contributor.authorTanaka, A. S.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:38:02Z
dc.date.available2016-01-24T12:38:02Z
dc.date.issued2005-09-01
dc.description.abstractBlood-sucking arthropods are vectors responsible for the transmission of several pathogens and parasites to vertebrate animals. the horn fly Haematobia irritans irritans (Diptera: Muscidae) and the tick Boophilus microplus are important hematophagous ecto-parasites that cause losses in cattle production. A serine protease inhibitor from a thorax extract of the fly H. irritans irritans (HiTI) was previously isolated, characterized and cloned. in the present study we described the expression, purification, and characterization of the recombinant HiTI (rHiTI) and its possible role in the control of different endogenous and bacterial proteases. rHiTI was successfully expressed using the pPIC9 expression vector with a yield of 4.2 mg/L of active rHiTI. the recombinant HiTI purified by affinity chromatography on trypsin-Sepharose had a molecular mass of 6.53 kDa as determined by LS-ESI mass spectrometry and inhibition constants (Kis) similar to those of native HiTI for bovine trypsin and human neutrophil elastase of 0.4 and 1.0 nM, respectively. Purified rHiTI also showed inhibitory activity against the trypsin-like enzyme of H. i. irritans using its possible natural substrates, fibrinogen and hemoglobin; and also inhibited the OmpT endoprotease of Escherichia coli using fluorogenic substrates. the present results confirm that HiTI may play a role in the control of fly endogenous proteases but also suggest a role in the inhibition of pathogen proteases. (c) 2005 Elsevier Inc. All rights reserved.en
dc.description.affiliationUNIFESP EPM, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.affiliationUNIFESP EPM, Dept Biofis, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUNIFESP EPM, Dept Bioquim, BR-04044020 São Paulo, Brazil
dc.description.affiliationUnifespUNIFESP EPM, Dept Biofis, BR-04044020 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent30-36
dc.identifierhttp://dx.doi.org/10.1016/j.exppara.2005.03.013
dc.identifier.citationExperimental Parasitology. San Diego: Academic Press Inc Elsevier Science, v. 111, n. 1, p. 30-36, 2005.
dc.identifier.doi10.1016/j.exppara.2005.03.013
dc.identifier.issn0014-4894
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/28446
dc.identifier.wosWOS:000231354500005
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofExperimental Parasitology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectflyen
dc.subjectserine protease inhibitoren
dc.subjectinnate immunityen
dc.subjectomp-t endoproteaseen
dc.titleThe role of HiTI, a serine protease inhibitor from Haematobia irritans irritans (Diptera : Muscidae) in the control of fly and bacterial proteasesen
dc.typeinfo:eu-repo/semantics/article
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