Impact of oxygen supply on surfactin biosynthesis using brewery waste as substrate

Zanutto, Talita Corrêa Nazareth; Zanutto, Conrado Planas; Maass, Danielle [UNIFESP]; Souza, Antônio Augusto Ulson de; Souza, Selene Maria de Arruda Guelli Ulson de

Impact of oxygen supply on surfactin biosynthesis using brewery waste as substrate

Arquivos

Impact of oxygen supply on surfactin biosynthesis using brewery waste as substrate.pdf(1.81 MB)

Data

2021-03-15

Autores

Zanutto, Talita Corrêa Nazareth

Zanutto, Conrado Planas

Maass, Danielle

Souza, Antônio Augusto Ulson de

Souza, Selene Maria de Arruda Guelli Ulson de

Tipo

Artigo

Resumo

This work reports surfactin production by B. subtilis ATCC 6051 in a stirred tank bioreactor, where trub was used as a carbon source. Trub is a residue from the brewing industry mainly composed of high-molar-mass proteins and reducing sugars, being a sustainable and low-cost substrate for bioprocess. Despite this, Trub has been little explored as a raw material in bioprocesses. Aiming to evaluate important factors in scale-up process, the impact of oxygen transfer coefficient (KLa) concerning volumetric productivity, and the effect of agitation (150–300 rpm) and aeration (0.5–1.5 vvm) on biosurfactant production by a Full Factorial design were evaluated. Only agitation presented a significant effect on surfactin concentration (SF), where SF was described by a linear regression model (R2 = 0.99). The biosurfactant showed high stability in relation to variations in temperature and pH. In addition, the foam collecting was an efficient method in recovering and enrichment of the product.