O potencial da luz ultravioleta na descontaminação de biofilme fúngico em lentes de contato gelatinosas
Data
2022-05-27
Autores
Rosa, Larissa Rigobeli da 
Orientadores
Cristovam, Priscila Cardoso
Tipo
Dissertação de mestrado
Título da Revista
ISSN da Revista
Título de Volume
Resumo
A utilização de Lentes de Contato, tanto para correção de problemas oculares
como para uso estético, pode ocasionar sérios problemas oculares em seus
usuários, como ceratites e hipersensibilidade a soluções destinadas a limpeza
das lentes. Entre as causas dessas complicações, está o mau uso dos produtos
disponíveis no mercado para a limpeza das lentes (que demandam tempo e
dedicação para que sua ação germicida seja alcançada), a toxicidade dos
reagentes presentes nesses produtos e a formação de biofilme aumentam o risco
das complicações oculares decorrente das infecções. A luz ultravioleta (UV) é
uma onda eletromagnética que carrega energia, está compreendida entre os
espectros de onda do raio-X. Seu potencial germicida é capaz de eliminar
microrganismos de superfícies bióticas como células e abióticas como o plástico.
O presente estudo tem como objetivo comparar o potencial da luz ultravioleta na
descontaminação de lentes de contato gelatinosas em relação a solução de
limpeza. Lentes de contato gelatinosas foram contaminadas com cepas
conhecidas de Candida albicans e Fusarium solani e posteriormente tratadas de
acordo com os grupos (n=5): solução limpeza (SL) ou luz ultravioleta (UVC).
Lentes contaminadas sem tratamento foram utilizadas como controle positivo
(CT+) e, lentes virgens sem contaminação ou tratamento foram utilizadas como
controle negativo (CT-). A formação de biofilme foi avaliada por microscopia
óptica e por coloração com Calcofluor White, a viabilidade dos microrganismos
foi avaliada pelo método de XTT. Além disso, medidas de diâmetros das lentes
de contatos foram obtidas por paquimetria, dioptrias por lensômetro, e análise
das propriedades termodinâmicas das LC por análise termogravimétrica. Os
resultados demonstraram que o uso isolado da luz Uv ou solução de limpeza não
foi 100% eficaz na descontaminação das lentes de contato, porém, a ação
germicida da luz Uv sobre o biofilme de Candida albicans e Fusarium solani
formado em lentes de contato gelatinosas apresentou ser mais efetiva em
comparação a solução de limpeza, sem induzir alteração das propriedades das
lentes de contato.
The use of Contact Lenses, both for correction of eye problems and for aesthetic use, can cause serious eye problems in its users, such as keratitis and hypersensitivity to solutions intended for lens cleaning. Among the causes of these complications is the misuse of products available on the market for cleaning lenses (which require time and dedication for their germicidal action to be achieved), the toxicity of the reagents present in these products and the formation of biofilm difficult and increases the risk of eye complications resulting from infections. Ultraviolet light (UV) is an electromagnetic wave that carries energy, it is included in the X-ray wave spectra. Its germicidal potential is capable of eliminating microorganisms from biotic surfaces such as cells and abiotic surfaces such as plastic. The present study aims to compare the potential of ultraviolet light in the decontamination of soft contact lenses in relation to the cleaning solution. Soft contact lenses were contaminated with known strains of Candida albicans and Fusarium solani and subsequently treated according to the groups (n=5): cleaning solution (SL) or ultraviolet light (UVC). Untreated contaminated lenses were used as a positive control (CT+) and virgin lenses without contamination or treatment were used as a negative control (CT-). Biofilm formation was evaluated by optical microscopy and Calcofluor White staining, viability of the microorganisms was evaluated by the XTT method. In addition, measurements of contact lens diameters were obtained by pachymetry, diopters by lensometer, and analysis of the thermodynamic properties of contact lenses by thermogravimetric analysis. The results showed that the isolated use of UV light or cleaning solution was not 100% effective in decontaminating contact lenses, however, the germicidal action of UV light on Candida albicans and Fusarium solani biofilm formed on soft contact lenses showed be more effective compared to cleaning solution, without inducing change in contact lens properties.
The use of Contact Lenses, both for correction of eye problems and for aesthetic use, can cause serious eye problems in its users, such as keratitis and hypersensitivity to solutions intended for lens cleaning. Among the causes of these complications is the misuse of products available on the market for cleaning lenses (which require time and dedication for their germicidal action to be achieved), the toxicity of the reagents present in these products and the formation of biofilm difficult and increases the risk of eye complications resulting from infections. Ultraviolet light (UV) is an electromagnetic wave that carries energy, it is included in the X-ray wave spectra. Its germicidal potential is capable of eliminating microorganisms from biotic surfaces such as cells and abiotic surfaces such as plastic. The present study aims to compare the potential of ultraviolet light in the decontamination of soft contact lenses in relation to the cleaning solution. Soft contact lenses were contaminated with known strains of Candida albicans and Fusarium solani and subsequently treated according to the groups (n=5): cleaning solution (SL) or ultraviolet light (UVC). Untreated contaminated lenses were used as a positive control (CT+) and virgin lenses without contamination or treatment were used as a negative control (CT-). Biofilm formation was evaluated by optical microscopy and Calcofluor White staining, viability of the microorganisms was evaluated by the XTT method. In addition, measurements of contact lens diameters were obtained by pachymetry, diopters by lensometer, and analysis of the thermodynamic properties of contact lenses by thermogravimetric analysis. The results showed that the isolated use of UV light or cleaning solution was not 100% effective in decontaminating contact lenses, however, the germicidal action of UV light on Candida albicans and Fusarium solani biofilm formed on soft contact lenses showed be more effective compared to cleaning solution, without inducing change in contact lens properties.
Descrição
Citação
ROSA, Larissa Rigobeli da. O potencial da luz ultravioleta na descontaminação de biofilme fúngico em lentes de contato gelatinosas. São Paulo, 2022. 56 f. Dissertação (Mestrado Profissional em Tecnologias e Atenção à Saúde) – Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, 2022.