Efeito do exercício aeróbico sobre o núcleo pulposo de ratos diabéticos
Data
2022-02-24
Autores
Kasinski, Silvana Kertzer 
Orientadores
Higa, Elisa Mieko Suemitsu
Tipo
Dissertação de mestrado
Título da Revista
ISSN da Revista
Título de Volume
Resumo
Introdução: A dor lombar tem sido associada à degeneração do núcleo pulposo (NP)
do disco intervertebral (DIV). Doenças crônicas, como o diabetes mellitus (DM),
acentuam a degeneração do DIV. A peroxidação lipídica causada por radicais livres e a
inflamação, por meio de mediadores como IL-6 e TNF-α, contribuem para a
degeneração do NP. Protocolos de exercícios aeróbicos têm mostrado resultados
eficazes no tratamento do DM e da lombalgia. Objetivo: O presente estudo teve como
objetivo analisar o impacto do exercício aeróbio sobre o processos inflamatório e o
estresse oxidativo no NP dos DIVs de ratos diabéticos. Materiais e métodos: Foram
incluídos 20 ratos Wistar uninefrectomizados e induzidos ao DM. Os ratos foram
alocados em quatro grupos: CTL+SE (controle sedentário); CTL+EX (controle sob
exercício); DM+SE (diabético sedentário); e DM+EX (diabético sob exercício). O
protocolo de exercícios consistiu em corridas por uma hora por dia, durante oito
semanas. Ao final do treinamento, foram feitas análises da função renal, por meio das
dosagens plasmáticas de ureia e creatinina, e da peroxidação lipídica, por meio de
dosagens TBARS (substâncias reativas ao ácido tiobarbitúrico). Os DIVs lombares
foram removidos para análise histológica e de imunohistoquímica para avaliar a
expressão de IL-6, IL-10, TNF-α e MDA (malondialdeído, substância derivada da
peroxidação lipídica). Resultados: A análise histológica do NP mostrou maior número
de células com núcleos normais em ratos DM+EX em comparação com ratos DM+SE
(p<0,01). Ratos DM+EX tiveram expressão diminuída de IL-6 e de TNF-alfa em
comparação com DM+SE (p<0,001 e p<0,05, respectivamente). Os níveis de creatinina
e TBARS de ratos DM+EX mostraram-se reduzidos em comparação com ratos DM+SE
(respectivamente p<0,001 e p<0,05). Conclusão: A atividade física regular mostrou
eficácia em atenuar as condições patológicas induzidas pelo diabetes no NP, pois
limitou o impacto da inflamação ao reduzir a expressão de IL-6 e TNF-α, preservando a
estrutura celular e tecidual. A atividade física aeróbica também mostrou eficácia em
reduzir a peroxidação lipídica e melhorar a função renal de ratos diabéticos.
Background: Low back pain has been associated with degeneration of the nucleus pulposus (NP) of the intervertebral disc (IVD). Chronic diseases, such as diabetes mellitus (DM), accentuate IVD degeneration. Lipid peroxidation caused by free radicals and inflammation, through mediators such as IL-6 and TNF-α, contribute to the degeneration of the NP. Aerobic exercise protocols have shown effective results in the treatment of DM and low back pain. Objective: The present study aimed to analyze the impact of aerobic exercise on the inflammatory process and oxidative stress in the NP of DIVs of diabetic rats. Material and methods: We included 20 uninephrectomized Wistar rats with induced DM. The rats were divided into four groups: CTL+SE (control sedentary); CTL+EX (control exercise); DM+SE (diabetic sedentary); and DM+EX (diabetic exercise). The exercise protocol consisted of running for one hour a day for eight weeks. At the end of training, analyses of renal function were performed by measuring plasma levels of urea and creatinine, and lipid peroxidation, through TBARS levels (thiobarbituric acid reactive substances). Lumbar IVDs were removed for histological and immunohistochemical analysis to assess the expression of IL-6, IL-10, TNF-α and MDA (malondialdehyde, a substance derived from lipid peroxidation). Results: Histological analysis of the NP showed a greater number of cells with normal nuclei in DM+EX rats compared to DM+SE rats (p<0.01). DM+EX rats had decreased expression of IL-6 and TNF-alpha compared to DM+SE (p<0.001 and p<0.05, respectively). The creatinine and TBARS levels of DM+EX rats were reduced compared to DM+SE rats (respectively p<0.001 and p<0.05). Conclusion: Regular physical activity showed efficacy to attenuate the pathological conditions induced by diabetes in NP, which decreased the impact of inflammation and by reducing the expression of IL-6 and TNF-α, and preserved NP cell and tissue structure. Aerobic physical activity also showed efficacy in reducing lipid peroxidation and improving renal function in diabetic rats.
Background: Low back pain has been associated with degeneration of the nucleus pulposus (NP) of the intervertebral disc (IVD). Chronic diseases, such as diabetes mellitus (DM), accentuate IVD degeneration. Lipid peroxidation caused by free radicals and inflammation, through mediators such as IL-6 and TNF-α, contribute to the degeneration of the NP. Aerobic exercise protocols have shown effective results in the treatment of DM and low back pain. Objective: The present study aimed to analyze the impact of aerobic exercise on the inflammatory process and oxidative stress in the NP of DIVs of diabetic rats. Material and methods: We included 20 uninephrectomized Wistar rats with induced DM. The rats were divided into four groups: CTL+SE (control sedentary); CTL+EX (control exercise); DM+SE (diabetic sedentary); and DM+EX (diabetic exercise). The exercise protocol consisted of running for one hour a day for eight weeks. At the end of training, analyses of renal function were performed by measuring plasma levels of urea and creatinine, and lipid peroxidation, through TBARS levels (thiobarbituric acid reactive substances). Lumbar IVDs were removed for histological and immunohistochemical analysis to assess the expression of IL-6, IL-10, TNF-α and MDA (malondialdehyde, a substance derived from lipid peroxidation). Results: Histological analysis of the NP showed a greater number of cells with normal nuclei in DM+EX rats compared to DM+SE rats (p<0.01). DM+EX rats had decreased expression of IL-6 and TNF-alpha compared to DM+SE (p<0.001 and p<0.05, respectively). The creatinine and TBARS levels of DM+EX rats were reduced compared to DM+SE rats (respectively p<0.001 and p<0.05). Conclusion: Regular physical activity showed efficacy to attenuate the pathological conditions induced by diabetes in NP, which decreased the impact of inflammation and by reducing the expression of IL-6 and TNF-α, and preserved NP cell and tissue structure. Aerobic physical activity also showed efficacy in reducing lipid peroxidation and improving renal function in diabetic rats.