Determination of levetiracetam in human plasma by dispersive liquid-liquid microextraction followed by gas chromatography-mass spectrometry

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dc.contributor.authorSteinhorst Alcantara, Greyce Kelly
dc.contributor.authorCalixto, Leandro Augusto [UNIFESP]
dc.contributor.authorBeraldo de Moraes, Luiz Alberto
dc.contributor.authorCosta Queiroz, Regina Helena
dc.contributor.authorMoraes de Oliveira, Anderson Rodrigo
dc.contributor.authorde Gaitani, Cristiane Masetto
dc.date.accessioned2019-01-21T10:29:45Z
dc.date.available2019-01-21T10:29:45Z
dc.date.issued2016
dc.description.abstractLevetiracetam (LEV) is an antiepileptic drug that is clinically effective in generalized and partial epilepsy syndromes. The use of this drug has been increasing in clinical practice and intra- or -interindividual variability has been exhibited for special population. For this reason, bioanalytical methods are required for drug monitoring in biological matrices. So this work presents a dispersive liquid-liquid microextraction method followed by gas chromatography-mass spectrometry (DLLME-GC-MS) for LEV quantification in human plasma. However, due to the matrix complexity a previous purification step is required. Unlike other pretreatment techniques presented in the literature, for the first time, a procedure employing ultrafiltration tubes Amicon (R) (10 kDa porous size) without organic solvent consumption was developed. GC-MS analyses were carried out using a linear temperature program, capillary fused silica column, and helium as the carrier gas. DLLME optimized parameters were type and volume of extraction and dispersing solvents, salt addition, and vortex agitation time. Under chosen parameters (extraction solvent: chloroform, 130 mu Len
dc.description.abstractdispersing solvent: isopropyl alcohol, 400 mu Len
dc.description.abstractno salt addition and no vortex agitation time), the method was completely validated and all parameters were in agreement with the literature recommendations. LEV was quantified in patient's plasma sample using less than 550 mu L of organic solvent.en
dc.description.affiliationDepartment of Pharmaceutical Sciences, Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, 14040-903 Ribeirão Preto, SP, Brazil
dc.description.affiliationDepartment of Exact and Earth Sciences, Institute of Environmental, Chemical and Pharmaceutical Sciences, Federal University of São Paulo, 09972-270 Diadema, SP, Brazil
dc.description.affiliationDepartament of Chemistry, Faculty of Philosophy, Sciences and Letters of Ribeirão Preto, University of São Paulo, 14040-901 Ribeirão Preto, SP, Brazil
dc.description.affiliationDepartment of Clinical Analysis, Toxicology and Food Science, Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, 14040-903 Ribeirão Preto, SP, Brazil
dc.description.affiliationUnifespDepartment of Exact and Earth Sciences, Institute of Environmental, Chemical and Pharmaceutical Sciences, Federal University of São Paulo, 09972-270 Diadema, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipSao Paulo Research Foundation (FAPESP) [2012/07210-8]
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
dc.description.sponsorshipIDFAPESP: 2012/07210-8
dc.identifierhttp://dx.doi.org/10.1155/2016/5976324
dc.identifier.citationJournal Of Analytical Methods In Chemistry. London, 2016.
dc.identifier.doi10.1155/2016/5976324
dc.identifier.fileWOS000386837800001.pdf
dc.identifier.issn2090-8865
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/49371
dc.identifier.wosWOS:000386837800001
dc.language.isoeng
dc.publisherUniv Sao Paulo, Inst Psiquiatria
dc.relation.ispartofJournal Of Analytical Methods In Chemistry
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectSolid-Phase Extractionen
dc.subjectHplc-Uven
dc.subjectAntiepileptic Drugsen
dc.subjectOrganic-Compoundsen
dc.subjectHuman Urineen
dc.subjectPerformanceen
dc.subjectWateren
dc.subjectSamplesen
dc.subjectZonisamideen
dc.subjectSerumen
dc.titleDetermination of levetiracetam in human plasma by dispersive liquid-liquid microextraction followed by gas chromatography-mass spectrometryen
dc.typeinfo:eu-repo/semantics/article
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