Characterization of Mycobacterium chelonae-Like Strains by Comparative Genomics

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dc.citation.volume8
dc.contributor.authorNogueira, Christiane L. [UNIFESP]
dc.contributor.authorde Almeida, Luiz G. P.
dc.contributor.authorMenendez, Maria C.
dc.contributor.authorGarcia, Maria J.
dc.contributor.authorDigiampietri, Luciano A.
dc.contributor.authorChimara, Erica
dc.contributor.authorCnockaert, Margo
dc.contributor.authorPalomino, Juan C.
dc.contributor.authorPortaels, Francoise
dc.contributor.authorMartin, Anandi
dc.contributor.authorVandamme, Peter
dc.contributor.authorLeao, Sylvia C. [UNIFESP]
dc.coverageLausanne
dc.date.accessioned2020-07-13T11:53:14Z
dc.date.available2020-07-13T11:53:14Z
dc.date.issued2017
dc.description.abstractIsolates of the Mycobacterium chelonae-M. abscessus complex are subdivided into four clusters (CHI to CHIV) in the INNO-LiPA (R) Mycobacterium spp DNA strip assay. A considerable phenotypic variability was observed among isolates of the CHII cluster. In this study, we examined the diversity of 26 CHII cluster isolates by phenotypic analysis, drug susceptibility testing, whole genome sequencing and single-gene analysis. Pairwise genome comparisons were performed using several approaches, including average nucleotide identity (ANI) and genome-to-genome distance (GGD) among others. Based on ANI and GGD the isolates were identified as M. chelonae (14 isolates), M. franklinii (2 isolates) and M. salmoniphium (1 isolate). The remaining 9 isolates were subdivided into three novel putative genomospecies. Phenotypic analyses including drug susceptibility testing, as well as whole genome comparison by TETRA and delta differences, were not helpful in separating the groups revealed by ANI and GGD. The analysis of standard four conserved genomic regions showed that rpoB alone and the concatenated sequences clearly distinguished the taxonomic groups delimited by whole genome analyses. In conclusion, the CHII INNO-LiPa is not a homogeneous clusteren
dc.description.abstracton the contrary, it is composed of closely related different species belonging to the M. chelonae-M. abscessus complex and also several unidentified isolates. The detection of these isolates, putatively novel species, indicates a wider inner variability than the presently known in this complex.en
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, Sao Paulo, Brazil
dc.description.affiliationLab Nacl Comp Cient, Petropolis, Brazil
dc.description.affiliationUniv Autonoma Madrid, Fac Med, Dept Med Prevent, Madrid, Spain
dc.description.affiliationUniv Sao Paulo, Escola Artes Ciencias & Humanidades, Sao Paulo, Brazil
dc.description.affiliationAdolfo Lutz Inst, Nucleo TB & Micobacterioses, Sao Paulo, Brazil
dc.description.affiliationUniv Ghent, Fac Sci, Microbiol Lab, Ghent, Belgium
dc.description.affiliationInst Trop Med, Dept Biomed Sci, Mycobacteriol Unit, Antwerp, Belgium
dc.description.affiliationUnifespUniv Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
dc.description.sponsorshipFAPESP
dc.description.sponsorshipIDFAPESP: 2011/18326-4
dc.description.sponsorshipIDFAPESP: 2012/13763-0
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.3389/fmicb.2017.00789
dc.identifier.citationFrontiers In Microbiology. Lausanne, v. 8, p. -, 2017.
dc.identifier.doi10.3389/fmicb.2017.00789
dc.identifier.fileWOS000400731200001.pdf
dc.identifier.issn1664-302X
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/54468
dc.identifier.wosWOS:000400731200001
dc.language.isoeng
dc.publisherFrontiers Media Sa
dc.relation.ispartofFrontiers In Microbiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectmycobacteriumen
dc.subjectM. chelonae-M. abscessus complexen
dc.subjectwhole genome sequencingen
dc.subjecttaxonomyen
dc.subjectidentificationen
dc.titleCharacterization of Mycobacterium chelonae-Like Strains by Comparative Genomicsen
dc.typeinfo:eu-repo/semantics/article
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