Leucine supplementation is anti-atrophic during paradoxical sleep deprivation in rats

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dc.citation.issue4
dc.citation.volume48
dc.contributor.authorSouza, Helton de Sá [UNIFESP]
dc.contributor.authorAntunes, Hanna Karen Moreira [UNIFESP]
dc.contributor.authorDattilo, Murilo [UNIFESP]
dc.contributor.authorLee, Kil Sun [UNIFESP]
dc.contributor.authorMônico-Neto, Marcos [UNIFESP]
dc.contributor.authorGiampá, Sara Quaglia de Campos [UNIFESP]
dc.contributor.authorPhillips, Stuart M.
dc.contributor.authorTufik, Sergio [UNIFESP]
dc.contributor.authorMello, Marco Tulio de [UNIFESP]
dc.coverageWien
dc.date.accessioned2020-07-22T13:23:18Z
dc.date.available2020-07-22T13:23:18Z
dc.date.issued2016
dc.description.abstractThe purpose of this study was to identify sleep deprivation-induced atrophy and the muscle-specific fiber types affected and to determine the effects of leucine supplementation on atrophy and pertinent portions of the pathways of muscle protein synthesis and degradation in rats. A total of 46 Wistar rats were distributed in four groups: control (CTL), leucine supplementation (LEU), sleep deprivation (SD), and leucine supplementation + sleep deprivation (LEU + SD). Leucine supplementation was by gavage (1.35 g/kg/daily), and the animals were subjected to SD for 96 h. Testosterone and corticosterone concentrations, along with proteins involved in protein synthesis and degradation and proteasome activity levels, were measured in the gastrocnemius (GA) muscle. Myosin ATPase staining was used to evaluate the different muscle fibers. After sleep deprivation, GA muscle and body masses decreased in the SD group compared to the CTL, LEU, and LEU + SD groups. There was no difference between groups in type I fiber cross-sectional area (CSA). The CSAs for type IIa fibers were lower in the SD and LEU + SD groups vs. the CTL and LEU groups, while the IIb fiber CSA was lower in the SD group vs. the CSAs in all other groups. The phospho (p)-Akt levels were lower in the SD and LEU + SD groups vs. the CTL and LEU groups. The p-mTORC1 levels were higher in the LEU, SD, and LEU + SD groups vs. the CTL group. The p-p70S6k levels were higher in the LEU and LEU + SD groups; the 4E-BP1 levels were higher in the SD and LEU + SD groups compared to those in the CTL and LEU groups, and the p-4E-BP1 levels were higher in the LEU and SD groups compared to those in the CTL group and even higher in the LEU + SD group compared to those in the LEU and SD groups. Ubiquitinated proteins, LC3, and p62/SQSTM, and proteasome activity levels were higher in the SD and LEU + SD groups vs. the LEU and CTL groups. Sleep deprivation led to the atrophy of IIa and IIb muscle fibers; however, leucine supplementation prevented muscle loss and type IIb fiber atrophy.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Psicobiol, BR-11015020 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Biociencias, Campus Baixada Santista,Rua Silva Jardim 136, BR-11015020 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Bioquim, BR-11015020 Sao Paulo, SP, Brazil
dc.description.affiliationUniv Fed Minas Gerais, Escola Educ Fis Fisioterapia & Terapia Ocupac, Belo Horizonte, MG, Brazil
dc.description.affiliationLab Interdisciplinar Fisiol & Exercicio LAIFE, Sao Paulo, SP, Brazil
dc.description.affiliationMcMaster Univ, Dept Kinesiol, Hamilton, ON, Canada
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Psicobiol, BR-11015020 Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Biociencias, Campus Baixada Santista,Rua Silva Jardim 136, BR-11015020 Sao Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Bioquim, BR-11015020 Sao Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.format.extent949-957
dc.identifierhttps://dx.doi.org/10.1007/s00726-015-2142-7
dc.identifier.citationAmino Acids. Wien, v. 48, n. 4, p. 949-957, 2016.
dc.identifier.doi10.1007/s00726-015-2142-7
dc.identifier.issn0939-4451
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/56161
dc.identifier.wosWOS:000372551100005
dc.language.isoeng
dc.publisherSpringer Wien
dc.relation.ispartofAmino Acids
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectmTORC1en
dc.subjectSkeletal muscleen
dc.subjectProtein synthesisen
dc.subjectProtein degradationen
dc.titleLeucine supplementation is anti-atrophic during paradoxical sleep deprivation in ratsen
dc.typeinfo:eu-repo/semantics/article
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