Critical involvement of Th1-related cytokines in renal injuries induced by ischemia and reperfusion

dc.contributor.authorPaiva, Vanessa Nunes de [UNIFESP]
dc.contributor.authorMonteiro, Rebecca M. M. [UNIFESP]
dc.contributor.authorMarques, Vilmar de Paiva
dc.contributor.authorCenedeze, Marcos Antonio [UNIFESP]
dc.contributor.authorTeixeira, Vicente de P. A.
dc.contributor.authorReis, Marlene A. dos
dc.contributor.authorPacheco-Silva, Alvaro [UNIFESP]
dc.contributor.authorCamara, Niels O. S. [UNIFESP]
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionHosp Israelita Albert Einstein
dc.contributor.institutionUniv Fed Triangulo Mineiro
dc.description.abstractRenal ischemia and reperfusion injury (IRI) is considered an inflammatory syndrome. To move forward in its pathogenesis, we exploited the role of several cytokines on renal damages triggered by IRI. Specifically to evaluate the role of Th1 immune profile in this system, IL-12, IFN-gamma, and IFN-gamma/IL-12 deficient (KO) mice on C57BL/6 background and their controls were subjected to IRI. in each group, blood and kidney samples were harvested. Renal function was evaluated by serum creatinine and renal morphometric analyses. Gene expression of IL-6 and HO-1 were also investigated by Q-PCR. IFN-gamma KO animals presented the highest impairment in renal function compared to controls. Conversely, IL-12 KO animals were absolutely protected and, in a lesser extent, IFN-gamma/IL-12 KO double knockout was also protected from IRI. Gene expression analyses showed higher expression of HO-1, a cytoprotective gene, and IL-6, a pro-inflammatory cytokine, in IFN-gamma deficient animals subjected to IRI. Our results confirm that Th1 related cytokines such as IL-12 and IFN-gamma are critically involved in renal ischemia and reperfusion injury. (C) 2008 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv São Paulo, Inst Biomed Sci, Dept Immunol, Div Immunol,Lab Transplantat Immunobiol, BR-05508900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, EPM, Dept Med, Clin & Expt Immunol Lab,Nephrol Div, São Paulo, Brazil
dc.description.affiliationHosp Israelita Albert Einstein, IEP, São Paulo, Brazil
dc.description.affiliationUniv Fed Triangulo Mineiro, Dept Pathol, Uberaba, MG, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, EPM, Dept Med, Clin & Expt Immunol Lab,Nephrol Div, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.identifier.citationInternational Immunopharmacology. Amsterdam: Elsevier B.V., v. 9, n. 6, p. 668-672, 2009.
dc.publisherElsevier B.V.
dc.relation.ispartofInternational Immunopharmacology
dc.rightsAcesso restrito
dc.subjectAcute renal failureen
dc.subjectIschemia and reperfusion injuryen
dc.subjectInterferon gammaen
dc.titleCritical involvement of Th1-related cytokines in renal injuries induced by ischemia and reperfusionen