Assessment of free fetal DNA concentration in maternal plasma during the first trimester of pregnancy: comparative study between EDTA and PPT tubes - pilot study

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dc.contributor.authorChadud, Carolina Schneider
dc.contributor.authorAraujo Junior, Edward [UNIFESP]
dc.contributor.authorMartinhago, Ciro Dresh
dc.contributor.authorMello Andari, Viviane Cristina
dc.contributor.authorTedesco, Giselle Darahem
dc.contributor.authorSilva Bussamra, Luiz Claudio [UNIFESP]
dc.contributor.authorAoki, Tsutomu
dc.contributor.institutionMed Coll Sci Santa Casa São Paulo FCMSCSP
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionRDO Med Diagnost
dc.date.accessioned2016-01-24T14:39:53Z
dc.date.available2016-01-24T14:39:53Z
dc.date.issued2015-01-01
dc.description.abstractObjective: To compare ethylenediamine tetraacetic acid (EDTA) tubes and plasma preparation tubes (PPT) for evaluating maternal plasma during the first trimester of pregnancy.Methods: A cross-sectional study was conducted on 24 male fetuses in women between 6 and 14 weeks of pregnancy. Blood samples (10 mL) were collected and stored in EDTA and PPT tubes. Subsequently, the samples were centrifuged and sent for free fetal DNA extraction by means of the polymerase chain reaction (PCR) technique. the reactions were performed in a real time PCR machine for detecting the amplification products. the genome region chosen for performing the PCR reactions was a target specific for the Y chromosome, in which the DYS-14 marker was amplified only when the DNA was of male sex. the free fetal DNA concentration was given by the threshold cycle (TC). To compare the tubes, the paired Student t-test was used.Results: the mean gestational age was 11.08 +/- 2.30 weeks (range: 6-14). the mean TC for PPT was 30.08 +/- 1.05 (range: 27.08-32.61) and for EDTA, 30.23 +/- 0.96 (range: 28.01-32.09), but without statistical significance (p = 0.357).Conclusion: We did not observe any statistically significant difference in free fetal DNA concentration between the EDTA and PPT tubes.en
dc.description.affiliationMed Coll Sci Santa Casa São Paulo FCMSCSP, Dept Obstet & Gynecol, São Paulo, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo UNIFESP, Dept Obstet, São Paulo, SP, Brazil
dc.description.affiliationRDO Med Diagnost, São Paulo, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo UNIFESP, Dept Obstet, São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent172-176
dc.identifierhttp://dx.doi.org/10.3109/14767058.2014.908843
dc.identifier.citationJournal of Maternal-fetal & Neonatal Medicine. London: Informa Healthcare, v. 28, n. 2, p. 172-176, 2015.
dc.identifier.doi10.3109/14767058.2014.908843
dc.identifier.issn1476-7058
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/38623
dc.identifier.wosWOS:000346922300011
dc.language.isoeng
dc.publisherInforma Healthcare
dc.relation.ispartofJournal of Maternal-fetal & Neonatal Medicine
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://informahealthcare.com/userimages/ContentEditor/1255620309227/Copyright_And_Permissions.pdf
dc.subjectEDTAen
dc.subjectfirst trimester of pregnancyen
dc.subjectfree fetal DNAen
dc.subjectPCRen
dc.subjectPPTen
dc.titleAssessment of free fetal DNA concentration in maternal plasma during the first trimester of pregnancy: comparative study between EDTA and PPT tubes - pilot studyen
dc.typeinfo:eu-repo/semantics/article
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