Detection of Colistin-Resistant MCR-1-Positive Escherichia coli by Use of Assays Based on Inhibition by EDTA and Zeta Potential

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dc.citation.issue12
dc.citation.volume55
dc.contributor.authorEsposito, Fernanda
dc.contributor.authorFernandes, Miriam R.
dc.contributor.authorLopes, Ralf
dc.contributor.authorMunoz, Maria
dc.contributor.authorSabino, Caetano P.
dc.contributor.authorCunha, Marcos P.
dc.contributor.authorSilva, Ketrin C.
dc.contributor.authorCayo, Rodrigo [UNIFESP]
dc.contributor.authorMartins, Willames M. B. S. [UNIFESP]
dc.contributor.authorMoreno, Andrea M.
dc.contributor.authorKnobl, Terezinha
dc.contributor.authorGales, Ana C. [UNIFESP]
dc.contributor.authorLincopan, Nilton
dc.coverageWashington
dc.date.accessioned2020-09-01T13:21:11Z
dc.date.available2020-09-01T13:21:11Z
dc.date.issued2017
dc.description.abstractThe emergence and rapid dissemination of colistin-resistant Escherichia coli carrying the plasmid-mediated mcr-1 gene have created an urgent need to develop specific screening methods. In this study, we evaluated four assays based on the inhibition of MCR-1 activity by EDTA: (i) a combined-disk test (CDT) comparing the inhibition zones of colistin and colistin (10 mu g) plus EDTA (100 mM); (ii) reduction of colistin MIC (CMR) in the presence of EDTA (80 mu g/ml); (iii) a modified rapid polymyxin Nordmann/Poirel test (MPNP); and (iv) alteration of zeta potential (R-ZP = ZP(+EDTA)/ZP(-EDTA)). We obtained encouraging results for the detection of MCR-1 in E. coli isolates recovered from human, food, and animal samples, using the following assay parameters: >= 3 mm difference in the inhibition zones between colistin disks without and with EDTA; >= 4-fold colistin MIC decrease in the presence of EDTA; R-ZP of >= 2.5; and the absence of metabolic activity and proliferation, indicated by unchanged color of phenol red in the presence of colistin-EDTA, in the MPNP test. In this regard, the CDT, CMR, R-ZP, and MPNP assays exhibited sensitivities of 96.7, 96.7, 95.1, and 96.7% and specificities of 89.6, 83.3, 100, and 100%, respectively, for detecting MCR-1-positive E. coli. Our results demonstrate that inhibition by EDTA and zeta potential assays may provide simple and inexpensive methods for the presumptive detection of MCR-1-producing E. coli isolates in human and veterinary diagnostic laboratories.en
dc.description.affiliationUniv Sao Paulo, Dept Clin Anal, Sch Pharm, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Sch Vet Med, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Escola Paulista Med, Dept Internal Med, Lab Alerta,Div Infect Dis, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Escola Paulista Med, Dept Internal Med, Lab Alerta,Div Infect Dis, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
dc.description.sponsorshipNational Council for Science and Technological Development (CNPq)
dc.description.sponsorshipIDFAPESP: 2016/08593-9
dc.description.sponsorshipIDFAPESP: 2015/13527-2
dc.description.sponsorshipIDCNPq: 462042/2014-6
dc.description.sponsorshipIDCNPq: 305535/2014-5
dc.description.sponsorshipIDCAPES: PNPD 20131991
dc.format.extent3454-3465
dc.identifierhttp://dx.doi.org/10.1128/JCM.00835-17
dc.identifier.citationJournal Of Clinical Microbiology. Washington, v. 55, n. 12, p. 3454-3465, 2017.
dc.identifier.doi10.1128/JCM.00835-17
dc.identifier.issn0095-1137
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/58116
dc.identifier.wosWOS:000416225200019
dc.language.isoeng
dc.publisherAmer Soc Microbiology
dc.relation.ispartofJournal Of Clinical Microbiology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectpolymyxinsen
dc.subjectcolistin resistanceen
dc.subjectcombined disk testen
dc.subjectpolymyxin NP testen
dc.subjectcharge modificationen
dc.titleDetection of Colistin-Resistant MCR-1-Positive Escherichia coli by Use of Assays Based on Inhibition by EDTA and Zeta Potentialen
dc.typeinfo:eu-repo/semantics/article
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