Bone marrow-derived mesenchymal stem cells versus adipose-derived mesenchymal stem cells for peripheral nerve regeneration

Página do item simplificado
dc.citation.issue1
dc.citation.volume13
dc.contributor.authorFernandes, Marcela [UNIFESP]
dc.contributor.authorValente, Sandra Gomes [UNIFESP]
dc.contributor.authorSabongi, Rodrigo Guerra [UNIFESP]
dc.contributor.authorGomes dos Santos, Joao Baptista [UNIFESP]
dc.contributor.authorLeite, Vilnei Mattioli [UNIFESP]
dc.contributor.authorUlrich, Henning
dc.contributor.authorNery, Arthur Andrade
dc.contributor.authorda Silva Fernandes, Maria Jose [UNIFESP]
dc.coverageMumbai
dc.date.accessioned2020-07-02T18:52:04Z
dc.date.available2020-07-02T18:52:04Z
dc.date.issued2018
dc.description.abstractStudies have confirmed that bone marrow-derived mesenchymal stem cells (MSCs) can be used for treatment of several nervous system diseases. However, isolation of bone marrow-derived MSCs (BMSCs) is an invasive and painful process and the yield is very low. Therefore, there is a need to search for other alterative stem cell sources. Adipose-derived MSCs (ADSCs) have phenotypic and gene expression profiles similar to those of BMSCs. The production of ADSCs is greater than that of BMSCs, and ADSCs proliferate faster than BMSCs. To compare the effects of venous grafts containing BMSCs or ADSCs on sciatic nerve injury, in this study, rats were randomly divided into four groups: sham (only sciatic nerve exposed), Matrigel (MGen
dc.description.abstractsciatic nerve injury + intravenous transplantation of MG vehicle), ADSCs (sciatic nerve injury + intravenous MG containing ADSCs), and BMSCs (sciatic nerve injury + intravenous MG containing BMSCs) groups. Sciatic functional index was calculated to evaluate the function of injured sciatic nerve. Morphologic characteristics of nerves distal to the lesion were observed by toluidine blue staining. Spinal motor neurons labeled with Fluoro-Gold were quantitatively assessed. Compared with sham-operated rats, sciatic functional index was lower, the density of small-diameter fibers was significantly increased, and the number of motor neurons significantly decreased in rats with sciatic nerve injury. Neither ADSCs nor BMSCs significantly improved the sciatic nerve function of rats with sciatic nerve injury, increased fiber density, fiber diameters, axonal diameters, myelin sheath thickness, and G ratios (axonal diameter/fiber diameter ratios) in the sciatic nerve distal to the lesion site. There was no significant difference in the number of spinal motor neurons among ADSCs, BMSCs and MG groups. These results suggest that neither BMSCs nor ADSCs provide satisfactory results for peripheral nerve repair when using MG as the conductor for engraftment.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Orthoped & Traumatol, Div Hand & Upper Limb Surg, Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Inst Chem, Dept Biochem, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Div Neurosci, Dept Neurol & Neurosurg, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Orthoped & Traumatol, Div Hand & Upper Limb Surg, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Div Neurosci, Dept Neurol & Neurosurg, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
dc.description.sponsorshipCAPES
dc.format.extent100-104
dc.identifierhttp://dx.doi.org/10.4103/1673-5374.224378
dc.identifier.citationNeural Regeneration Research. Mumbai, v. 13, n. 1, p. 100-104, 2018.
dc.identifier.doi10.4103/1673-5374.224378
dc.identifier.fileWOS000425047900016.pdf
dc.identifier.issn1673-5374
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/53863
dc.identifier.wosWOS:000425047900016
dc.language.isoeng
dc.publisherMedknow Publications & Media Pvt Ltd
dc.relation.ispartofNeural Regeneration Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectnerve regenerationen
dc.subjectmesenchymal stem cellsen
dc.subjectadipose-derived mesenchmal stem cellsen
dc.subjectsciatic nerveen
dc.subjectMatrigelen
dc.subjectsciatic functional indexen
dc.subjectneural regenerationen
dc.titleBone marrow-derived mesenchymal stem cells versus adipose-derived mesenchymal stem cells for peripheral nerve regenerationen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
WOS000425047900016.pdf
Tamanho:
867.76 KB
Formato:
Adobe Portable Document Format
Descrição:
Baixar
Coleções
EPM - Artigos