Mycobacterium tuberculosis epitope-specific interferon-gamma production in healthy Brazilians reactive and non-reactive to tuberculin skin test

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dc.contributor.authorMaciel da Silva, Bosco Christiano [UNIFESP]
dc.contributor.authorRios Grassi, Maria Fernanda
dc.contributor.authorCoutinho, Raimundo
dc.contributor.authorMoreira Mascarenhas, Rita Elizabeth
dc.contributor.authorOlavarria, Viviana Nilla
dc.contributor.authorCoutinho-Borgo, Adriana [UNIFESP]
dc.contributor.authorKalil, Jorge [UNIFESP]
dc.contributor.authorCunha-Neto, Edecio [UNIFESP]
dc.contributor.authorFonseca, Simone Goncalves [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionFundacao Oswaldo Cruz
dc.contributor.institutionEscola Bahiana Med & Saude Publ
dc.contributor.institutionInst Invest Immunol
dc.date.accessioned2016-01-24T14:38:13Z
dc.date.available2016-01-24T14:38:13Z
dc.date.issued2014-12-01
dc.description.abstractThe interferon (IFN)-gamma response to peptides can be a useful diagnostic marker of Mycobacterium tuberculosis (MTB) latent infection. We identified promiscuous and potentially protective CD4(+) T-cell epitopes from the most conserved regions of MTB antigenic proteins by scanning the MTB antigenic proteins GroEL2, phosphate-binding protein 1 precursor and 19 kDa antigen with the TEPITOPE algorithm. Seven peptide sequences predicted to bind to multiple human leukocyte antigen (HLA)-DR molecules were synthesised and tested with IFN-gamma enzyme-linked immunospot (ELISPOT) assays using peripheral blood mononuclear cells (PBMCs) from 16 Mantoux tuberculin skin test (TST)-positive and 16 TST-negative healthy donors. Eighty-eight percent of TST-positive donors responded to at least one of the peptides, compared to 25% of TST-negative donors. Each individual peptide induced IFN-gamma production by PBMCs from at least 31% of the TST-positive donors. the magnitude of the response against all peptides was 182 +/- 230 x 10(6) IFN-gamma spot forming cells (SFC) among TST-positive donors and 36 +/- 62 x 10(6) SFC among TST-negative donors (p = 0.007). the response to GroEL2 (463-477) was only observed in the TST-positive group. This combination of novel MTB CD4 T-cell epitopes should be tested in a larger cohort of individuals with latent tuberculosis (TB) to evaluate its potential to diagnose latent TB and it may be included in ELISPOT-based IFN-gamma assays to identify individuals with this condition.en
dc.description.affiliationUniversidade Federal de São Paulo, Fac Med, Inst Coracao, Immunol Lab, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Fac Med, Dept Med, Div Imunol Clin & Alergia, São Paulo, Brazil
dc.description.affiliationFundacao Oswaldo Cruz, Lab Avancado Saude Publ, Salvador, BA, Brazil
dc.description.affiliationEscola Bahiana Med & Saude Publ, Salvador, BA, Brazil
dc.description.affiliationInst Invest Immunol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Fac Med, Inst Coracao, Immunol Lab, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Fac Med, Dept Med, Div Imunol Clin & Alergia, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent999-1004
dc.identifierhttp://dx.doi.org/10.1590/0074-0276140193
dc.identifier.citationMemorias Do Instituto Oswaldo Cruz. Rio de Janeiro, Rj: Fundaco Oswaldo Cruz, v. 109, n. 8, p. 999-1004, 2014.
dc.identifier.doi10.1590/0074-0276140193
dc.identifier.fileS0074-02762014000700970.pdf
dc.identifier.issn0074-0276
dc.identifier.scieloS0074-02762014000700970
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/38486
dc.identifier.wosWOS:000347528000005
dc.language.isoeng
dc.publisherFundaco Oswaldo Cruz
dc.relation.ispartofMemorias Do Instituto Oswaldo Cruz
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMycobacterium tuberculosisen
dc.subjectepitopesen
dc.subjectIFN-gamma ELISPOTen
dc.subjectlatent tuberculosisen
dc.subjectMantoux tuberculin skin testen
dc.titleMycobacterium tuberculosis epitope-specific interferon-gamma production in healthy Brazilians reactive and non-reactive to tuberculin skin testen
dc.typeinfo:eu-repo/semantics/article
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