Surface Molecules Released by Trypanosoma cruzi Metacyclic Forms Downregulate Host Cell Invasion

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dc.citation.issue8
dc.citation.volume10
dc.contributor.authorClemente, Tatiana Mordente [UNIFESP]
dc.contributor.authorCortez, Cristian [UNIFESP]
dc.contributor.authorNovaes, Antonio da Silva [UNIFESP]
dc.contributor.authorYoshida, Nobuko [UNIFESP]
dc.coverageSan Francisco
dc.date.accessioned2020-08-14T13:43:58Z
dc.date.available2020-08-14T13:43:58Z
dc.date.issued2016
dc.description.abstractBackground The question whether metacylic trypomastigote (MT) forms of different T. cruzi strains differentially release surface molecules, and how they affect host cell invasion, remains to be fully clarified. We addressed that question using T. cruzi strains that differ widely in the ability to invade cells. Methodology/Principal Findings Metacyclic forms were incubated at 37 degrees C for 1 h in complete D10 medium or in nutrient-deprived PBS containing Ca2+ and Mg2+ (PBS++). The conditioned medium (CM), collected after parasite centrifugation, was used for cell invasion assays and Western blot analysis, using monoclonal antibodies directed to gp82 and gp90, the MT surface molecules that promote and negatively regulate invasion, respectively. CM of poorly invasive G strain (G-CM) contained high amounts of gp90 and gp82, either in vesicles or as soluble molecules. CM of highly invasive CL strain (CL-CM) contained gp90 and gp82 at very low levels. HeLa cells were incubated for 1 h with CL strain MT in D10, in absence or in the presence of G-CM or CL-CM. Parasite invasion was significantly inhibited by G-CM, but not by CL-CM. As G strain MT invasion rate in D10 is very low, assays with this strain were performed in PBS++, which induces invasion-promoting lysosome-spreading. G-CM, but not CL-CM, significantly inhibited G strain internalization, effect that was counteracted by preincubating G-CM with an anti-gp90 monoclonal antibody or anti-gp82 polyclonal antibody that do not recognize live MT. G strain CM generated in PBS++ contained much lower amounts of gp90 and gp82 as compared to CM produced in D10, and exhibited lower inhibitory effect on host cell invasion. Conclusion/Significance Our data suggest that the surface molecules spontaneously released by MT impair parasite-host cell interaction, gp82 presumably competing with the molecule expressed on MT surface for the host cell receptor, and gp90 further contributing to down modulate invasion.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Dept Med, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Med, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq)
dc.description.sponsorshipIDFAPESP: 11/51475-3
dc.description.sponsorshipIDCNPq: 300578/2010-5
dc.format.extent-
dc.identifierhttp://dx.doi.org/10.1371/journal.pntd.0004883
dc.identifier.citationPlos Neglected Tropical Diseases. San Francisco, v. 10, n. 8, p. -, 2016.
dc.identifier.doi10.1371/journal.pntd.0004883
dc.identifier.issn1935-2735
dc.identifier.urihttps://repositorio.unifesp.br/handle/11600/57468
dc.identifier.wosWOS:000382390800027
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPlos Neglected Tropical Diseases
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleSurface Molecules Released by Trypanosoma cruzi Metacyclic Forms Downregulate Host Cell Invasionen
dc.typeinfo:eu-repo/semantics/article
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