Effect of antipsychotic drugs on gene expression in the prefrontal cortex and nucleus accumbens in the spontaneously hypertensive rat (SHR)

dc.contributor.authorSantoro, Marcos Leite [UNIFESP]
dc.contributor.authorOta, Vanessa Kiyomi [UNIFESP]
dc.contributor.authorStilhano, Roberta Sessa [UNIFESP]
dc.contributor.authorSilva, Patricia Natalia [UNIFESP]
dc.contributor.authorSantos, Camila Mauricio [UNIFESP]
dc.contributor.authorDiana, Mariana Cepollaro [UNIFESP]
dc.contributor.authorGadelha, Ary [UNIFESP]
dc.contributor.authorBressan, Rodrigo Affonseca [UNIFESP]
dc.contributor.authorMelaragno, Maria Isabel [UNIFESP]
dc.contributor.authorHan, Sang Won [UNIFESP]
dc.contributor.authorAbilio, Vanessa Costhek [UNIFESP]
dc.contributor.authorBelangero, Sintia Iole [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T14:37:40Z
dc.date.available2016-01-24T14:37:40Z
dc.date.issued2014-08-01
dc.description.abstractAntipsychotic drugs (APDs) are the standard treatment for schizophrenia. the therapeutic effect of these drugs is dependent upon the dopaminergic D2 blockade, but they also modulate other neurotransmitter pathways. the exact mechanisms underlying the clinical response to APDs are not fully understood. in this study, we compared three groups of animals for the expression of 84 neurotransmitter genes in the prefrontal cortex (PFC) and nucleus accumbens (NAcc). Each group was treated with a different APD (risperidone, clozapine or haloperidol), and with a non-treated group of spontaneously hypertensive rats (SHRs), which is an animal model for schizophrenia. This study also explored whether or not differential expression was regulated by DNA methylation in the promoter region (PR). in the clozapine group, we found that Chrng was downregulated in the NAcc and six geneswere downregulated in the PFC. in the haloperidol group, Brs3 and Glra1 were downregulated, as was Drd2 in the clozapine group and Drd3, Galr3 and Gabrr1 in the clozapine and haloperidol groups. We also encountered four hypermethylated CG sites in the Glra1 PR, as well as three in the risperidone group and another in the haloperidol group, when compared to non-treated rats. Following the APD treatment, the gene expression results revealed the involvement of genes that had not previously been described, in addition to the activity of established genes. the investigation of the involvement of these novel genes can lead to better understanding about the specific mechanisms of action of the individual APDs studied. (C) 2014 Elsevier B. V. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo UNIFESP, Dept Morphol & Genet, Div Genet, BR-04023900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Psychiat, LiNC Interdisciplinary Lab Clin Neurosci, BR-05039032 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo UNIFESP, Dept Biophys, BR-04044010 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo UNIFESP, Dept Pharmacol, BR-04039032 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo UNIFESP, Dept Morphol & Genet, Div Genet, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Psychiat, LiNC Interdisciplinary Lab Clin Neurosci, BR-05039032 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo UNIFESP, Dept Biophys, BR-04044010 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo UNIFESP, Dept Pharmacol, BR-04039032 São Paulo, Brazil
dc.description.provenanceMade available in DSpace on 2016-01-24T14:37:40Z (GMT). No. of bitstreams: 0 Previous issue date: 2014-08-01en
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIDFAPESP: 2010/08968-6
dc.description.sponsorshipIDFAPESP: 2010/13859-1
dc.format.extent163-168
dc.identifierhttp://dx.doi.org/10.1016/j.schres.2014.05.015
dc.identifier.citationSchizophrenia Research. Amsterdam: Elsevier B.V., v. 157, n. 1-3, p. 163-168, 2014.
dc.identifier.doi10.1016/j.schres.2014.05.015
dc.identifier.issn0920-9964
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/38056
dc.identifier.wosWOS:000341314100026
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofSchizophrenia Research
dc.rightsAcesso restrito
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectSpontaneously hypertensive ratsen
dc.subjectAntipsychoticen
dc.subjectGene expressionen
dc.subjectDNA methylationen
dc.subjectGlra1en
dc.subjectBrs3en
dc.subjectDrd2en
dc.subjectDrd3en
dc.subjectGalr3en
dc.subjectGabrr1en
dc.titleEffect of antipsychotic drugs on gene expression in the prefrontal cortex and nucleus accumbens in the spontaneously hypertensive rat (SHR)en
dc.typeArtigo
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