Leishmania (L.) amazonensis: Fusion between parasitophorous vacuoles in infected bone-marrow derived mouse macrophages

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dc.contributor.authorReal, F. [UNIFESP]
dc.contributor.authorPouchelet, M.
dc.contributor.authorRabinovitch, M. [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionINSERM
dc.date.accessioned2016-01-24T13:49:48Z
dc.date.available2016-01-24T13:49:48Z
dc.date.issued2008-05-01
dc.description.abstract[Leishmania (L.)] amazonensis amastigotes reside in macrophages within spacious parasitophorous vacuoles (PVs) which may contain numerous parasites. After sporadic fusion events were detected by time-lapse cinemicrography, PV fusion was examined in two different models. in single infections, it was inferred from the reduction in PV numbers per cell. in a reinfection model, macrophages infected with unlabeled amastigotes were reinfected with GFP-transfected- or carboxyfluorescein diacetate succinimidyl ester-labeled parasites, and fusion was detected by the colocalization of labeled and unlabeled amastigotes in the same PVs. the main findings were: (1) as expected, fusion frequency increased with the multiplicity of infection; (2) most fusion events took place in the first 24 h of infection or reinfection, prior to the multiplication of incoming parasites; (3) resident and incoming parasites multiplied at similar rates in fused PVs. the model should be useful in studies of parasite and host cell factors and mechanisms involved in PV fusogenicity. (C) 2007 Elsevier Inc. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationINSERM, Serv Microcinematog, F-78110 Le Vesinet, France
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent15-23
dc.identifierhttp://dx.doi.org/10.1016/j.exppara.2007.12.013
dc.identifier.citationExperimental Parasitology. San Diego: Academic Press Inc Elsevier Science, v. 119, n. 1, p. 15-23, 2008.
dc.identifier.doi10.1016/j.exppara.2007.12.013
dc.identifier.issn0014-4894
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/30640
dc.identifier.wosWOS:000255728800003
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofExperimental Parasitology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectprotozoaen
dc.subjectLeishmaniaen
dc.subjectLeishmania (L.) amazonensisen
dc.subjectamastigoteen
dc.subjectmacrophagesen
dc.titleLeishmania (L.) amazonensis: Fusion between parasitophorous vacuoles in infected bone-marrow derived mouse macrophagesen
dc.typeinfo:eu-repo/semantics/article
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