Development of a prototype immunochromatographic test for rapid diagnosis of respiratory adenovirus infection

dc.contributor.authorPaulini, Inarei [UNIFESP]
dc.contributor.authorSiqueira-Silva, Joselma
dc.contributor.authorThomaz, Luciana
dc.contributor.authorRocha, Leticia
dc.contributor.authorHarsi, Charlotte
dc.contributor.authorBellei, Nancy [UNIFESP]
dc.contributor.authorGranato, Celso [UNIFESP]
dc.date.accessioned2019-08-19T11:48:28Z
dc.date.available2019-08-19T11:48:28Z
dc.date.issued2017
dc.description.abstractHuman adenoviruses comprise an important group of etiologie agents that are responsible for various diseases in adults and children, such as respiratory, ocular, gastroenteric, and urinary infections. In immunocompromised and organ-transplanted individuals, these agents can cause generalized infections. Rapid diagnostic methods for detecting these infectious agents are not widely available.& para;& para;The aim of this work was to produce monoclonal and polyclonal anti-adenovirus antibodies to be used in a rapid diagnostic test for respiratory infections.& para;& para;Adenovirus hexons were satisfactorily purified by ultracentrifugation and chromatography. After virus purification, anti-hexon monoclonal antibodies were produced and characterized, following classical methods. Antibodies were specific for adenoviruses 2,3,5, and 41. The proposed immunochromatographic test was standardized using colloidal gold.& para;& para;The standardization of the rapid test was sufficient to detect adenovirus antigens (in nasopharyngeal lavage samples) with sensitivity of 100% and specificity of 85% when compared to direct immunofluorescence.& para;& para;The immunochromatographic assay prototype was sufficiently sensitive to detect B (3), C (2 and 5), and F (41) adenovirus samples. Although based on preliminary data, the test demonstrated the same performance as direct immunofluorescence, but with the advantage of being a point-of-care test. Further studies are still needed to confirm its effectiveness in clinical practice. (C) 2017 Sociedade Brasueira de Infectologia. Published by Elsevier Editera Ltda.en
dc.description.affiliationUniv Fed São Paulo, Dept Med, Lab Virol, Disciplina Infectol, São Paulo, SP, Brazil
dc.description.affiliationUniv Fed São Paulo, Inst Biociencias, Dept Microbiol, Lab Adenovirus, São Paulo, SP, Brazil
dc.description.affiliationInst Biociencias, Dept Microbiol, Lab Micol, São Paulo, SP, Brazil
dc.description.affiliationInst Butantan, Lab Bacteriol, São Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed São Paulo, Dept Med, Lab Virol, Disciplina Infectol, São Paulo, SP, Brazil
dc.description.affiliationUnifespUniv Fed São Paulo, Inst Biociencias, Dept Microbiol, Lab Adenovirus, São Paulo, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIDFAPESP: 2011/50100-6
dc.format.extent500-506
dc.identifierhttp://dx.doi.org/10.1016/j.bjid.2017.03.023
dc.identifier.citationBrazilian Journal Of Infectious Diseases. Rio De Janeiro, v. 21, n. 5, p. 500-506, 2017.
dc.identifier.doi10.1016/j.bjid.2017.03.023
dc.identifier.fileWOS000414276300002.pdf
dc.identifier.issn1413-8670
dc.identifier.scieloS1413-86702017000500500
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/51258
dc.identifier.wosWOS:000414276300002
dc.language.isoeng
dc.publisherElsevier Brazil
dc.rightsAcesso aberto
dc.subjectAdenovirusen
dc.subjectMonoclonal antibodiesen
dc.subjectImmunochromatographic testen
dc.titleDevelopment of a prototype immunochromatographic test for rapid diagnosis of respiratory adenovirus infectionen
dc.title.alternativeDevelopment of a prototype immunochromatographic test for rapid diagnosis of respiratory adenovirus infectionpt
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