Experimental paracoccidioidomycosis in high and low antibody responder mice of Selection IV-A

dc.contributor.authorSoares, Angela Maria Victoriano de Campos
dc.contributor.authorRezkallah-Iwasso, Maria Thereza
dc.contributor.authorOliveira, S. L.
dc.contributor.authorPeraçoli, Maria Terezinha Serrão
dc.contributor.authorMontenegro, Mário Rubens Guimarães
dc.contributor.authorMusatti, Chloe Camba [UNIFESP]
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.description.abstractHigh (H) and low (L) responder mice were selected for their ability to produce antibodies against sheep and human erythrocytes (Selection IV-A). In this selection, the difference in antibody responsiveness between H and L lines (HIV-A and LIV-A mice, respectively) was shown to depend mainly on macrophage function. The more rapid catabolism of antigens by macrophages in L mice has been suggested as the main cause of the low antibody production. Due to this high macrophage activity, L animals have been described as more resistant than H animals to intracellular pathogens. These animals were utilized as an experimental model of paracoccidioidomycosis. HIV-A and LIV-A mice were infected with Paracoccidioides brasiliensis by the intravenous route. As expected, H mice were more susceptible to P. brasiliensis with a shorter survival time and higher levels of specific antibodies when compared to L mice. Contrasting with the survival time, the lungs, spleen and liver from H mice showed typical nodular granulomas containing epithelioid and giant cells and few fungi. On the other hand, in LN-A mice, the lesions of these organs were characterized by looser granulomas with irregular borders and the presence of a large number of fungi, However, the adrenal gland showed different lesion patterns. In H mice these lesions were extensive and characterized by loose granulomas with numerous fungi, while in LIV-A mice the lesions were small and limited to the cortex. Moreover the HIV-A mice presented higher levels of serum corticosterone when compared to LIV-A ones. The higher susceptibility of H mice could be attributed to the extensive lesions of the adrenal glands. These results suggest the use of the H line from the IV-A Selection as an experimental model for further studies of adrenal involvement in paracoccidioidomycosis.en
dc.description.affiliationUNESP, Biosci Inst, Dept Microbiol & Immunol, BR-18618000 Sao Paulo, Brazil
dc.description.affiliationUNESP, Sch Med, Dept Pathol, Botucatu, SP, Brazil
dc.description.affiliationUNIFESP, Dept Pediat, Sao Paulo, Brazil
dc.description.affiliationUnifespUNIFESP, Dept Pediat, Sao Paulo, Brazil
dc.description.provenanceMade available in DSpace on 2018-06-18T11:03:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2000-08-01en
dc.description.sourceWeb of Science
dc.identifier.citationMedical Mycology. Oxford: B I O S Scientific Publishers Ltd, v. 38, n. 4, p. 309-315, 2000.
dc.publisherB I O S Scientific Publishers Ltd
dc.relation.ispartofMedical Mycology
dc.rightsAcesso restrito
dc.subjectadrenal glanden
dc.subjectmurine experimental paracoccidioidomycosisen
dc.titleExperimental paracoccidioidomycosis in high and low antibody responder mice of Selection IV-Aen