Heparin modulates the expression of genes encoding pro and anti-apoptotic proteins in endothelial cells exposed to intestinal ischemia and reperfusion in rats

Página do item simplificado
dc.contributor.authorTaha, Murched Omar [UNIFESP]
dc.contributor.authorMiranda-Ferreira, Regiane [UNIFESP]
dc.contributor.authorTaha, Nabiha Saadi Abrahão [UNIFESP]
dc.contributor.authorMonteiro, Hugo Pequeno [UNIFESP]
dc.contributor.authorCaricati-Neto, Afonso [UNIFESP]
dc.contributor.authorFagundes, Djalma José [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-06-14T13:47:12Z
dc.date.available2015-06-14T13:47:12Z
dc.date.issued2014-07-01
dc.description.abstractPURPOSE: To investigate if expression of genes encoding pro and anti-apoptotic proteins in the rat enteric endothelial cells stimulated by intestinal ischemia followed by reperfusion (IR) can be modified by treatment with heparin (HP).METHODS: Eighteen adult Wistar rats were divided in three groups: sham group submitted to laparotomy only (SG), ischemia followed by reperfusion group (IRG); ischemia followed by reperfusion plus pretreatment with HP 100 mg.kg-1 (IRG+HP). Ischemia was performed by clamping of the superior mesenteric artery. After 60 min of ischemia, metal clamps were removed for reperfusion for 120 min. Gene expression of encoding pro (Casp1, Casp6, Casp3, Cflar, Fas and Pgl) and anti-apoptotic (Bcl2, Bcl2l1 and Naip2) proteins in rat enteric endothelial cells was evaluated by PCR microarray method.RESULTS: Compared to rat endothelial cells of SG, the expression of pro-apoptotic genes was up-regulated in IRG while anti-apoptotic genes were down-regulated. In contrast, the expression of anti-apoptotic genes in IRG+HP was up-regulated while pro-apoptotic genes was down-regulated compared to SG.CONCLUSION: The attenuation by heparin of intestinal ischemia-reperfusion previously demonstrated in rodents could be related with ability of this drug to stimulate and reduce gene expression of encoding anti and pro-apoptotic proteins, respectively.en
dc.description.affiliationFederal University of São Paulo Department of Surgery
dc.description.affiliationUNIFESP Pharmacology Department
dc.description.affiliationUNIFESP Gynecology Department
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Department of Biochemistry
dc.description.affiliationUniversidade Federal de São Paulo (UNIFESP) Department of Surgery
dc.description.affiliationUnifespUNIFESP, Department of Surgery
dc.description.affiliationUnifespUNIFESP, Pharmacology Department
dc.description.affiliationUnifespUNIFESP, Gynecology Department
dc.description.affiliationUnifespUNIFESP, Department of Biochemistry
dc.description.affiliationUnifespUNIFESP, Department of Surgery
dc.description.sourceSciELO
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent445-449
dc.identifierhttp://dx.doi.org/10.1590/S0102-86502014000700006
dc.identifier.citationActa Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 29, n. 7, p. 445-449, 2014.
dc.identifier.doi10.1590/S0102-86502014000700006
dc.identifier.fileS0102-86502014000700445.pdf
dc.identifier.issn0102-8650
dc.identifier.scieloS0102-86502014000700445
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/8497
dc.identifier.wosWOS:000340835400006
dc.language.isoeng
dc.publisherSociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.relation.ispartofActa Cirurgica Brasileira
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectIschemiaen
dc.subjectReperfusion Injuryen
dc.subjectEndothelial Cellsen
dc.subjectGene Expression Profilingen
dc.subjectHeparinen
dc.subjectRatsen
dc.titleHeparin modulates the expression of genes encoding pro and anti-apoptotic proteins in endothelial cells exposed to intestinal ischemia and reperfusion in ratsen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
S0102-86502014000700445.pdf
Tamanho:
238.75 KB
Formato:
Adobe Portable Document Format
Descrição:
Baixar
Coleções
EPM - Artigos