Shiga toxin-producing Escherichia coli in drinking water supplies of north Parana State, Brazil

Página do item simplificado
dc.contributor.authorLascowski, K. M. S. [UNIFESP]
dc.contributor.authorGuth, B. E. C. [UNIFESP]
dc.contributor.authorMartins, F. H.
dc.contributor.authorRocha, S. P. D.
dc.contributor.authorIrino, K.
dc.contributor.authorPelayo, J. S.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Estadual de Londrina (UEL)
dc.contributor.institutionAdolfo Lutz Inst
dc.date.accessioned2016-01-24T14:31:33Z
dc.date.available2016-01-24T14:31:33Z
dc.date.issued2013-04-01
dc.description.abstractAim To determine the occurrence and characteristics of Shiga toxin-producing Escherichia coli (STEC) in drinking water supplies treated and untreated. Methods and Results Drinking water samples (n=1850) were collected from 41 municipalities in the north of Parana State between February 2005 and January 2006. Escherichia coli isolates (n=300) were recovered from water and investigated for the presence of virulence markers related to STEC by PCR. STEC isolates recovered were then characterized for both phenotypic and genotypic traits. A total of 12 isolates (11 from untreated water and one from treated water) were positive for stx, including five positive for both stx1 and stx2, two positive for stx1 and five positive for stx2. None of the STEC isolates contained eae, but other virulence genes were observed such as ehxA (100%), saa (100%), lpfAO113 (75%), iha (42%), subAB (25%) and cdtV (8%). Multidrug resistance was identified in 25% of the STEC isolates. the 12 STEC isolates belonged to seven distinct serotypes and pulsed-field gel electrophoresis typing revealed the presence of two clusters and two clones in this region. Conclusion Drinking water, especially from untreated water supplies, can be source of STEC strains potentially pathogenic for humans. Significance and Impact of the Study the investigation of the drinking water supplies for pathogenic E.coli, as STEC, may be useful to prevent waterborne outbreaks.en
dc.description.affiliationUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.affiliationUniv Estadual Londrina, Dept Microbiol, BR-86051980 Londrina, PR, Brazil
dc.description.affiliationAdolfo Lutz Inst, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent1230-1239
dc.identifierhttp://dx.doi.org/10.1111/jam.12113
dc.identifier.citationJournal of Applied Microbiology. Hoboken: Wiley-Blackwell, v. 114, n. 4, p. 1230-1239, 2013.
dc.identifier.doi10.1111/jam.12113
dc.identifier.issn1364-5072
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/36184
dc.identifier.wosWOS:000316572200032
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal of Applied Microbiology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjectantibioticsen
dc.subjectresistanceen
dc.subjectdrinking wateren
dc.subjectpulsed-field gel electrophoresisen
dc.subjectShiga toxin-producing Escherichia colien
dc.subjectvirulence markersen
dc.titleShiga toxin-producing Escherichia coli in drinking water supplies of north Parana State, Brazilen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
EPM - Artigos