Phytochemical analysis of hydroethanolic extract of turnera diffusa willd and evaluation of its effects on astrocyte cell death

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dc.contributor.authorBezerra, Andreia Gomes [UNIFESP]
dc.contributor.authorNegri, Giuseppina [UNIFESP]
dc.contributor.authorDuarte-Almeida, Joaquim Mauricio
dc.contributor.authorSmaili, Soraya Soubhi [UNIFESP]
dc.contributor.authorCarlini, Elisaldo Araujo [UNIFESP]
dc.date.accessioned2019-01-21T10:29:46Z
dc.date.available2019-01-21T10:29:46Z
dc.date.issued2016
dc.description.abstractObjective: To evaluate the phytochemical composition of hydroethanolic extracts from powdered aerial parts of Turnera diffusa Willd (Turneraceaeen
dc.description.abstractT. diffusa), as well as its toxicity in astrocytes. Methods: Chemical analyses of hydroethanolic extract from powdered aerial parts of T. diffusa were carried out using HPLC-DAD-ESI-MS/MS. In vitro assays using astrocytes culture were performed to evaluate cell death. Results: Flavone-C, O-diglycosides, such as, luteolin-8-C-[6-deoxy-2-O-rhamnosyl]-xylo-hexos-3-uloside, apigenin-8-C-[ 6-deoxy-2-O-rhamnosyl]-xylo-hexos-3-uloside and apigenin-7-O-6"-p-coumaroylglucoside were the main compounds found in this hydroethanolic extract. Concentration time-effect demonstrated the toxicity of this extract at a concentration of 1,000 mu g/mL in astrocyte culture, after 6 and 24 hours of incubation. Conclusion: In phytochemical analyses, important antioxidants (mainly flavonoids) were observed. T. diffusa extracts presented cytotoxic effect in high concentrations, leading to increased cell death in astrocyte culture.en
dc.description.affiliationUniversidade Federal de São Paulo, São Paulo, SP, Brasil
dc.description.affiliationUniversidade Federal de São João del-Rei, Divinópolis, MG, Brasil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Rua Napoleao de Barros 925, BR-04024002 Sao Paulo, SP, Brazil.
dc.description.sourceWeb of Science
dc.description.sponsorshipAssociacao Fundo de Incentivo a Psicofarmacologia (AFIP)
dc.description.sponsorshipCentro Brasileiro de Informacoes sobre Drogas Psicotropicas (CEBRID)
dc.description.sponsorshipCoordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
dc.description.sponsorshipFundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)
dc.format.extent56-63
dc.identifierhttp://dx.doi.org/10.1590/S1679-45082016AO3386
dc.identifier.citationEinstein-Sao Paulo. Sao paulo sp, v. 14, n. 1, p. 56-63, 2016.
dc.identifier.doi10.1590/S1679-45082016AO3386
dc.identifier.fileS1679-45082016000100056.pdf
dc.identifier.issn1679-4508
dc.identifier.scieloS1679-45082016000100056
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/49379
dc.identifier.wosWOS:000385715800012
dc.language.isoeng
dc.publisherElsevier Science Bv
dc.relation.ispartofEinstein-Sao Paulo
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCognition/Drug Effectsen
dc.subjectPlant Extracts/Toxicityen
dc.subjectProtective Agents/Toxicityen
dc.subjectAntioxidantsen
dc.subjectCell Death/Drug EffectsPerformance Liquid-Chromatographyen
dc.subjectQuadrupole Mass-Spectrometryen
dc.subjectUltraviolet Uv Radiationen
dc.subjectIn-Vitro Modelen
dc.subjectPhenolic-Compoundsen
dc.subjectElectrospray-Ionizationen
dc.subjectAntioxidant Propertiesen
dc.subjectAcid-Derivativesen
dc.subjectOxidative Stressen
dc.subjectQuality-Controlen
dc.titlePhytochemical analysis of hydroethanolic extract of turnera diffusa willd and evaluation of its effects on astrocyte cell deathen
dc.titleAnálise fitoquímica do extrato hidroetanólico de Turnera diffusa Willd e avaliação de seus efeitos na morte de astrócitospt
dc.typeinfo:eu-repo/semantics/article
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