Phytochemical analysis of hydroethanolic extract of turnera diffusa willd and evaluation of its effects on astrocyte cell death
Data
2016
Autores
Bezerra, Andreia Gomes 
Negri, Giuseppina
Smaili, Soraya Soubhi
Carlini, Elisaldo Araujo
Tipo
Artigo
Título da Revista
ISSN da Revista
Título de Volume
Resumo
Objective: To evaluate the phytochemical composition of hydroethanolic extracts from powdered aerial parts of Turnera diffusa Willd (Turneraceae
T. diffusa), as well as its toxicity in astrocytes. Methods: Chemical analyses of hydroethanolic extract from powdered aerial parts of T. diffusa were carried out using HPLC-DAD-ESI-MS/MS. In vitro assays using astrocytes culture were performed to evaluate cell death. Results: Flavone-C, O-diglycosides, such as, luteolin-8-C-[6-deoxy-2-O-rhamnosyl]-xylo-hexos-3-uloside, apigenin-8-C-[ 6-deoxy-2-O-rhamnosyl]-xylo-hexos-3-uloside and apigenin-7-O-6"-p-coumaroylglucoside were the main compounds found in this hydroethanolic extract. Concentration time-effect demonstrated the toxicity of this extract at a concentration of 1,000 mu g/mL in astrocyte culture, after 6 and 24 hours of incubation. Conclusion: In phytochemical analyses, important antioxidants (mainly flavonoids) were observed. T. diffusa extracts presented cytotoxic effect in high concentrations, leading to increased cell death in astrocyte culture.
T. diffusa), as well as its toxicity in astrocytes. Methods: Chemical analyses of hydroethanolic extract from powdered aerial parts of T. diffusa were carried out using HPLC-DAD-ESI-MS/MS. In vitro assays using astrocytes culture were performed to evaluate cell death. Results: Flavone-C, O-diglycosides, such as, luteolin-8-C-[6-deoxy-2-O-rhamnosyl]-xylo-hexos-3-uloside, apigenin-8-C-[ 6-deoxy-2-O-rhamnosyl]-xylo-hexos-3-uloside and apigenin-7-O-6"-p-coumaroylglucoside were the main compounds found in this hydroethanolic extract. Concentration time-effect demonstrated the toxicity of this extract at a concentration of 1,000 mu g/mL in astrocyte culture, after 6 and 24 hours of incubation. Conclusion: In phytochemical analyses, important antioxidants (mainly flavonoids) were observed. T. diffusa extracts presented cytotoxic effect in high concentrations, leading to increased cell death in astrocyte culture.
Descrição
Citação
Einstein-Sao Paulo. Sao paulo sp, v. 14, n. 1, p. 56-63, 2016.