Chemotactic signal transduction and phosphate metabolism as adaptive strategies during citrus canker induction by Xanthomonas citri

Página do item simplificado
dc.contributor.authorMoreira, Leandro Marcio
dc.contributor.authorFacincani, Agda Paula
dc.contributor.authorFerreira, Cristiano Barbalho
dc.contributor.authorFerreira, Rafael Marine
dc.contributor.authorTiraboshi Ferro, Maria Ines
dc.contributor.authorGozzo, Fabio Cesar
dc.contributor.authorFranco de Oliveira, Julio Cezar [UNIFESP]
dc.contributor.authorFerro, Jesus Aparecido
dc.contributor.authorSoares, Marcia Regina
dc.contributor.institutionUniv Fed Ouro Preto
dc.contributor.institutionUNESP Univ Estadual Paulista
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade Federal do Rio de Janeiro (UFRJ)
dc.date.accessioned2016-01-24T14:40:09Z
dc.date.available2016-01-24T14:40:09Z
dc.date.issued2015-03-01
dc.description.abstractThe genome of Xanthomonas citri subsp. Citri strain 306 pathotype A (Xac) was completely sequenced more than 10 years; to date, few studies involving functional genomics Xac and its host compatible have been developed, specially related to adaptive events that allow the survival of Xac within the plant. Proteomic analysis of Xac showed that the processes of chemotactic signal transduction and phosphate metabolism are key adaptive strategies during the interaction of a pathogenic bacterium with its plant host. the results also indicate the importance of a group of proteins that may not be directly related to the classical virulence factors, but that are likely fundamental to the success of the initial stages of the infection, such as methyl-accepting chemotaxis protein (Mcp) and phosphate specific transport (Pst). Furthermore, the analysis of the mutant of the gene pstB which codifies to an ABC phosphate transporter subunit revealed a complete absence of citrus canker symptoms when inoculated in compatible hosts. We also conducted an in silico analysis which established the possible network of genes regulated by two-component systems PhoPQ and PhoBR (related to phosphate metabolism), and possible transcriptional factor binding site (TFBS) motifs of regulatory proteins PhoB and PhoP, detaching high degree of conservation of PhoB TFBS in 84 genes of Xac genome. This is the first time that chemotaxis signal transduction and phosphate metabolism were therefore indicated to be fundamental to the process of colonization of plant tissue during the induction of disease associated with Xanthomonas genus bacteria.en
dc.description.affiliationUniv Fed Ouro Preto, Dept Ciencias Biol, Inst Ciencias Exatas & Biol, Ouro Preto, MG, Brazil
dc.description.affiliationUniv Fed Ouro Preto, Nucleo Pesquisas Ciencias Biol NUPEB, Ouro Preto, MG, Brazil
dc.description.affiliationUNESP Univ Estadual Paulista, Dept Tecnol, Fac Ciencias Agr & Vet Jaboticabal, São Paulo, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Inst Quim, UNICAMP, Campinas, SP, Brazil
dc.description.affiliationUniversidade Federal de São Paulo UNIFESP, Dept Ciencias Biol, Diadema, SP, Brazil
dc.description.affiliationUniv Fed Rio de Janeiro, Dept Bioquim, Inst Quim, Rio de Janeiro, RJ, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo UNIFESP, Dept Ciencias Biol, Diadema, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.description.sponsorshipIDFAPESP: 04/02006-7
dc.description.sponsorshipIDFAPEMIG: FAPEMIG-CBB-APQ-04425-10
dc.format.extent197-210
dc.identifierhttp://dx.doi.org/10.1007/s10142-014-0414-z
dc.identifier.citationFunctional & Integrative Genomics. Heidelberg: Springer Heidelberg, v. 15, n. 2, p. 197-210, 2015.
dc.identifier.doi10.1007/s10142-014-0414-z
dc.identifier.issn1438-793X
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/38813
dc.identifier.wosWOS:000351397700007
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofFunctional & Integrative Genomics
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dc.subjectXanthomonas adaptationen
dc.subjectChemotaxis signal transductionen
dc.subjectPhosphate metabolismen
dc.subjectTwo-component systems PhoBR and PhoPQen
dc.subjectVirulenceen
dc.titleChemotactic signal transduction and phosphate metabolism as adaptive strategies during citrus canker induction by Xanthomonas citrien
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
ICAQF - Artigos