Immunostimulatory DNA from Paracoccidioides brasiliensis acts as T-helper 1 promoter in susceptible mice

Página do item simplificado
dc.contributor.authorSouza, Marta Cristina [UNIFESP]
dc.contributor.authorCorrea, Mariangela [UNIFESP]
dc.contributor.authorAlmeida, Sandro Rogério de [UNIFESP]
dc.contributor.authorLopes, Jose Daniel [UNIFESP]
dc.contributor.authorCamargo, Zoilo Pires de [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2016-01-24T12:31:29Z
dc.date.available2016-01-24T12:31:29Z
dc.date.issued2001-10-01
dc.description.abstractTh1 immune responses afford protection against some pathogens like the fungus R brasiliensis (P.b.), ethiological agent of Paracoccidioidomycosis (PCM). It is well known that nonmethylated CpG sequences from bacterial DNA have immunomodulatory properties and can be used as a Th1-promoting adjuvant. By analyzing the available gene sequences of P.b. we observed a high number of unmethylated CpG dinucleotides. in a murine model of the PCM infection, the isogenic mouse strain known to be susceptible presents a predominant Th2 pattern. in order to access the possibility of the genomic DNA to act as a Th1-promoting adjuvant, in vitro assays were made and indicated a significant increase in phagocytosis when the macrophages were stimulated with DNA from Rb. and in vivo assays of a decreased production of antibodies antigp43, the main antigen of the PCM system. the analysis of the antibody isotypes and the cytokine production suggested a Th1 modulation in the susceptible animals. Thus, when mice were infected with fungus plus synthetic oligodeoxynucleotide (ODN), made from the available sequence of gp43, a decrease in the fungus dissemination was observed. Results herein described suggest that genomic DNA from Rb. could have a immunostimulatory function as a Th-1-promoting adjuvant in susceptible mice.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Microbiol Immunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent348-356
dc.identifierhttp://dx.doi.org/10.1046/j.1365-3083.2001.00937.x
dc.identifier.citationScandinavian Journal of Immunology. Oxford: Blackwell Science Ltd, v. 54, n. 4, p. 348-356, 2001.
dc.identifier.doi10.1046/j.1365-3083.2001.00937.x
dc.identifier.issn0300-9475
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/26630
dc.identifier.wosWOS:000171128100002
dc.language.isoeng
dc.publisherBlackwell Science Ltd
dc.relation.ispartofScandinavian Journal of Immunology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleImmunostimulatory DNA from Paracoccidioides brasiliensis acts as T-helper 1 promoter in susceptible miceen
dc.typeinfo:eu-repo/semantics/article
Arquivos
Coleções
EPM - Artigos