Structural features of forming and developing blood capillaries of the enamel organ of rat molar tooth germs observed by light and electron microscopy

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dc.contributor.authorManzke, E.
dc.contributor.authorKatchburian, E.
dc.contributor.authorFaria, F. P.
dc.contributor.authorFreymuller, E.
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Vale Itajai
dc.date.accessioned2016-01-24T12:38:01Z
dc.date.available2016-01-24T12:38:01Z
dc.date.issued2005-09-01
dc.description.abstractThe process of vascularization of the enamel organ, a unique epithelial structure, occurs when the tooth germ is fully developed, i.e., at the onset of dentinogenesis. Although the three-dimensional organization of the capillaries has been previously investigated, the structural features underlying the formation of the new capillaries remains poorly understood. Thus, in the hope of better understanding the mechanism of formation of the stellate reticulum capillaries, upper first molar tooth germs of newborn and 3-day-old rats were fixed in glutaraldehyde-formaldehyde and processed for light and electron microscopy. Our results showed that blood capillaries are initially in close proximity to the outer enamel epithelium. Between and intercalated with the capillaries are round/ovoid clusters of cells, some of which are vacuolated, closely apposed to the outer enamel epithelium. the outer enamel epithelium is not a continuous layer, but exhibits gaps between the cells. This suggests that the capillaries penetrate the enamel organ through these gaps, since no invagination of the epithelium was observed. the presence of a cluster of cells containing vacuoles suggests that vasculogenesis is taking place. Images showing loss of the basal lamina, proliferation of endothelial cells, presence of filopodia and lateral sprouting suggests that angiogenesis is also occurring. Thus, neoformation of capillaries of the molar enamel organ of rat seems to occur simultaneously by mechanisms of vasculogenesis and angiogenesis.en
dc.description.affiliationUniversidade Federal de São Paulo, Electron Microscopy Ctr, Sch Med, BR-04023900 São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Morphol, Sch Med, BR-04023900 São Paulo, Brazil
dc.description.affiliationUniv Vale Itajai, Hlth Sci Ctr, Itajai, SC, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Electron Microscopy Ctr, Sch Med, BR-04023900 São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Morphol, Sch Med, BR-04023900 São Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent335-342
dc.identifierhttp://dx.doi.org/10.1002/jmor.10363
dc.identifier.citationJournal of Morphology. Malden: Wiley-Blackwell, v. 265, n. 3, p. 335-342, 2005.
dc.identifier.doi10.1002/jmor.10363
dc.identifier.issn0362-2525
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/28441
dc.identifier.wosWOS:000231690300008
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal of Morphology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.subjectrat molaren
dc.subjectenamel organen
dc.subjectvasculogenesisen
dc.subjectangiogenesisen
dc.subjectultrastructureen
dc.titleStructural features of forming and developing blood capillaries of the enamel organ of rat molar tooth germs observed by light and electron microscopyen
dc.typeinfo:eu-repo/semantics/article
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