Improving typeability of multiple bacterial species using pulsed-field gel electrophoresis and thiourea
| dc.contributor.author | Silbert, S. | |
| dc.contributor.author | Boyken, L. | |
| dc.contributor.author | Hollis, R. J. | |
| dc.contributor.author | Pfaller, M. A. | |
| dc.contributor.institution | Universidade Federal de São Paulo (UNIFESP) | |
| dc.contributor.institution | Univ Iowa | |
| dc.date.accessioned | 2016-01-24T12:34:08Z | |
| dc.date.available | 2016-01-24T12:34:08Z | |
| dc.date.issued | 2003-12-01 | |
| dc.description.abstract | Although pulsed-field gel electrophoresis is considered the gold standard technique for molecular typing, typeability may not be excellent for some bacterial species because of DNA degradation. Previous reports suggest that the addition of thiourea in the gel buffer can improved the typeability for some species. in the present study, 66 Gram-negative strains (seven species) known to be affected by DNA degradation and four control strains were evaluated by PFGE with and without the addition of 50 mug/M of thiourea to the buffer used in the electrophoresis. Macrorestriction patterns were obtained for all K. pneumoniae, S. marcescens, P. aeruginosa, and Salmonella spp., for 95.4% of E. coli, and for 50% of E. cloacae strains from the gels performed in the buffer with throurea. However, typeability was not improved for Acinetobacter spp. the range of non-typeable species for which thiourea can limit the problem of DNA degradation is considerably wider than described in previous publications. (C) 2003 Elsevier Inc. All rights reserved. | en |
| dc.description.affiliation | Universidade Federal de São Paulo, Disciplina Doencas Infecciosas & Parasitarias, Lab Especial Microbiol Clin, São Paulo, Brazil | |
| dc.description.affiliation | Univ Iowa, Coll Med, Dept Pathol, Mol Epidemiol & Fungus Testing Lab, Iowa City, IA 52242 USA | |
| dc.description.affiliationUnifesp | Universidade Federal de São Paulo, Disciplina Doencas Infecciosas & Parasitarias, Lab Especial Microbiol Clin, São Paulo, Brazil | |
| dc.description.source | Web of Science | |
| dc.format.extent | 619-621 | |
| dc.identifier | http://dx.doi.org/10.1016/S0732-8893(03)00164-0 | |
| dc.identifier.citation | Diagnostic Microbiology and Infectious Disease. New York: Elsevier B.V., v. 47, n. 4, p. 619-621, 2003. | |
| dc.identifier.doi | 10.1016/S0732-8893(03)00164-0 | |
| dc.identifier.issn | 0732-8893 | |
| dc.identifier.uri | http://repositorio.unifesp.br/handle/11600/27500 | |
| dc.identifier.wos | WOS:000187897500012 | |
| dc.language.iso | eng | |
| dc.publisher | Elsevier B.V. | |
| dc.relation.ispartof | Diagnostic Microbiology and Infectious Disease | |
| dc.rights | info:eu-repo/semantics/restrictedAccess | |
| dc.rights.license | http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy | |
| dc.subject | PFGE | en |
| dc.subject | thiourea | en |
| dc.subject | molecular typing | en |
| dc.title | Improving typeability of multiple bacterial species using pulsed-field gel electrophoresis and thiourea | en |
| dc.type | info:eu-repo/semantics/article |