Evaluation of the metabolism of glycosaminoglycans in patients with interstitial cystis

dc.contributor.authorLucon, Marcos
dc.contributor.authorMartins, Joao Roberto
dc.contributor.authorLeite, Kátia Ramos Moreira [UNIFESP]
dc.contributor.authorSoler, Roberto
dc.contributor.authorNader, Helena Bonciani [UNIFESP]
dc.contributor.authorSrougi, Miguel [UNIFESP]
dc.contributor.authorBruschini, Homero
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2015-06-14T13:46:51Z
dc.date.available2015-06-14T13:46:51Z
dc.date.issued2014-01-01
dc.description.abstractIntroduction: Painful bladder syndrome/interstitial cystitis (PBS/IC) pathogenesis is not fully known, but evidence shows that glycosaminoglycans (GAG) of bladder urothelium can participate in its genesis. The loss of these compounds facilitates the contact of urine compounds with deeper portions of bladder wall triggering an inflammatory process. We investigated GAG in urine and tissue of PBS/IC and pure stress urinary incontinence (SUI) patients to better understand its metabolism. Materials and Methods: Tissue and urine of 11 patients with PBS/IC according to NIDDK criteria were compared to 11 SUI patients. Tissue samples were analyzed by histological, immunohistochemistry and immunofluorescence methods. Statistical analysis were performed using t Student test and Anova, considering significant when p < 0.05. Results: PBS/IC patients had lower concentration of GAG in urine when compared to SUI (respectively 0.45 ± 0.11 x 0.62 ± 0.13 mg/mg creatinine, p < 0.05). However, there was no reduction of the content of GAG in the urothelium of both groups. Immunofluorescence showed that PBS/IC patients had a stronger staining of TGF-beta, decorin (a proteoglycan of chondroitin/dermatan sulfate), fibronectin and hyaluronic acid. Conclusion: the results suggest that GAG may be related to the ongoing process of inflammation and remodeling of the dysfunctional urothelium that is present in the PBS/IC.en
dc.description.affiliationUniversity of São Paulo Medical Schoo Division of Urology
dc.description.affiliationFederal University of São Paulo Division of Urology
dc.description.affiliationFederal University of São Paulo Division of Molecular Biology
dc.description.affiliationUnifespUNIFESP, Division of Urology
dc.description.affiliationUnifespUNIFESP, Division of Molecular Biology
dc.description.sourceSciELO
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent72-79
dc.identifierhttp://dx.doi.org/10.1590/S1677-5538.IBJU.2014.01.11
dc.identifier.citationInternational braz j urol. Sociedade Brasileira de Urologia, v. 40, n. 1, p. 72-79, 2014.
dc.identifier.doi10.1590/S1677-5538.IBJU.2014.01.11
dc.identifier.fileS1677-55382014000100072.pdf
dc.identifier.issn1677-5538
dc.identifier.scieloS1677-55382014000100072
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/8164
dc.identifier.wosWOS:000335430800012
dc.language.isoeng
dc.publisherSociedade Brasileira de Urologia
dc.relation.ispartofInternational braz j urol
dc.rightsAcesso aberto
dc.subjectCystitis, Interstitialen
dc.subjectGlycosaminoglycansen
dc.subjectHyaluronic Aciden
dc.titleEvaluation of the metabolism of glycosaminoglycans in patients with interstitial cystisen
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