MONOCLONAL-ANTIBODY CAPTURE ENZYME-IMMUNOASSAY FOR DETECTION OF PARACOCCIDIOIDES-BRASILIENSIS ANTIBODIES IN PARACOCCIDIOIDOMYCOSIS

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dc.contributor.authorCamargo, Zoilo Pires de [UNIFESP]
dc.contributor.authorGesztesi, Jean-Luc [UNIFESP]
dc.contributor.authorSaraiva, Ernesto Carlos de Oliveira [UNIFESP]
dc.contributor.authorTaborda, Carlos Pelleschi [UNIFESP]
dc.contributor.authorVicentini, A. P. [UNIFESP]
dc.contributor.authorLopes, Jose Daniel [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.date.accessioned2018-06-15T17:58:34Z
dc.date.available2018-06-15T17:58:34Z
dc.date.issued1994-10-01
dc.description.abstractFour murine monoclonal antibodies (MAbs 17C, 21A, 21F, and 32B) raised against the 43-kDa glycoprotein of Paracoccidioides brasiliensis were tested in a capture enzyme immunoassay (EIA) for the detection of specific human anti-gp43 immunoglobulin G in patients with paracoccidioidomycosis (PCM). All MAbs reacted similarly in the assay. These MAbs, which detected anti-gp43 at levels of as low as 500 pg/ml, were demonstrated to specifically recognize at least two different epitopes in gp43 binding assays. Specific antibodies in the sera of patients with active PCM were detected at dilutions of as high as 1:819,200, and the reactivities of patient sera, as measured by optical densities, were found to be significantly higher than those of control sera. The comparison between classical ELISA and our capture enzyme immunoassay showed that both sensitivity and specificity were greatly improved by the latter. These MAbs represent the first specific reagents to P. brasiliensis described for use in serological tests for PCM.en
dc.description.affiliationESCOLA PAULISTA MED,DISCIPLINA BIOL CELULAR,RUA BOTUCATU 862,8 ANDAR,BR-04023062 SAO PAULO,BRAZIL
dc.description.affiliationUnifespESCOLA PAULISTA MED,DISCIPLINA BIOL CELULAR,RUA BOTUCATU 862,8 ANDAR,BR-04023062 SAO PAULO,BRAZIL
dc.description.sourceWeb of Science
dc.format.extent2377-2381
dc.identifierhttp://jcm.asm.org/content/32/10/2377
dc.identifier.citationJournal Of Clinical Microbiology. Washington: Amer Soc Microbiology, v. 32, n. 10, p. 2377-2381, 1994.
dc.identifier.issn0095-1137
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/44288
dc.identifier.wosWOS:A1994PG91200007
dc.language.isoeng
dc.publisherAmer Soc Microbiology
dc.relation.ispartofJournal Of Clinical Microbiology
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.titleMONOCLONAL-ANTIBODY CAPTURE ENZYME-IMMUNOASSAY FOR DETECTION OF PARACOCCIDIOIDES-BRASILIENSIS ANTIBODIES IN PARACOCCIDIOIDOMYCOSISen
dc.typeinfo:eu-repo/semantics/article
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