A Novel Monoclonal Antibody Against the C-terminus of beta-Tubulin Recognizes Endocytic Organelles in Trypanosoma cruzi

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dc.contributor.authorCornejo, Alberto [UNIFESP]
dc.contributor.authorOliveira, Claudio Rogerio de [UNIFESP]
dc.contributor.authorWuertele, Martin [UNIFESP]
dc.contributor.authorChung, Janete [UNIFESP]
dc.contributor.authorHilpert, Kai
dc.contributor.authorSchenkman, Sergio [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionForschungszentrum Karlsruhe
dc.date.accessioned2018-06-18T11:27:15Z
dc.date.available2018-06-18T11:27:15Z
dc.date.issued2012-06-01
dc.description.abstractMicrotubule cytoskeleton is a dynamic structure involved in the maintenance of eukaryote cell shape, motion of cilia and flagellum, and intracellular movement of vesicles and organelles. Many antibodies against tubulins have been described, most of them against the C-terminal portion, which is exposed at the outside of the microtubules. By generating a novel set of monoclonal antibodies against the cytoskeleton of Trypanosoma cruzi, a flagellate protozoan that causes Chagas' disease, we selected a clone (mAb 3G4) that recognizes beta-tubulin. The epitope for mAb 3G4 was mapped by pepscan to a highly conserved sequence motif found between alpha-helices 11 and 12 of the C-terminus of beta-tubulin in eukaryotes. It labels vesicular structures in both T. cruzi and mammalian cells, colocalizing respectively with a major cysteine protease (Cruzipain) and lysosome associated protein (LAMP2) respectively, but it does not label regular microtubules on these cellular models. We propose that the epitope recognized by mAb 3G4 is exposed only in a form of tubulin associated with endosomes.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, BR-04023062 Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, Inst Ciencia & Tecnol, BR-12231280 Sao Jose Dos Campos, Brazil
dc.description.affiliationForschungszentrum Karlsruhe, KIT, Inst Biol Interfaces, D-76021 Karlsruhe, Germany
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, BR-04023062 Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Inst Ciencia & Tecnol, BR-12231280 Sao Jose Dos Campos, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent636-643
dc.identifierhttp://dx.doi.org/10.2174/092986612800494075
dc.identifier.citationProtein And Peptide Letters. Sharjah: Bentham Science Publ Ltd, v. 19, n. 6, p. 636-643, 2012.
dc.identifier.doi10.2174/092986612800494075
dc.identifier.issn0929-8665
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/45147
dc.identifier.wosWOS:000304442400009
dc.language.isoeng
dc.publisherBentham Science Publ Ltd
dc.relation.ispartofProtein And Peptide Letters
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.subjectTubulinen
dc.subjectlysosomeen
dc.subjectendosomeen
dc.subjectepitopeen
dc.subjectmonoclonal antibodyen
dc.subjectTrypanosoma cruzien
dc.titleA Novel Monoclonal Antibody Against the C-terminus of beta-Tubulin Recognizes Endocytic Organelles in Trypanosoma cruzien
dc.typeinfo:eu-repo/semantics/article
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