Involvement of the NF-kappa B/p50/Bcl-3 complex in response to antiangiogenic therapy in a mouse model of metastatic renal cell carcinoma

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dc.contributor.authorBraga, Marina de Souza [UNIFESP]
dc.contributor.authorSilva Paiva, Katiucia Batista da
dc.contributor.authorFoguer, Karen [UNIFESP]
dc.contributor.authorBarbosa Chaves, Karen Cristina [UNIFESP]
dc.contributor.authorLima, Larissa de Sa
dc.contributor.authorScavone, Cristoforo
dc.contributor.authorBellini, Maria Helena [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionIPEN CNEN
dc.date.accessioned2016-01-24T14:37:48Z
dc.date.available2016-01-24T14:37:48Z
dc.date.issued2014-09-01
dc.description.abstractRenal cell carcinoma (RCC) represents approximately 2-3% of human malignancies. Nuclear transcription factor kappa B (NF-kappa B) is composed of a family of transcription factors that have been associated with the development and progression of RCC. Endostatin (ES) is a fragment of collagen XVIII that possesses antiangiogenic activity. in this study, we evaluated the expression of NF-kappa B in metastatic tumor cells from animals treated with ES. Balb/c-bearing Renca-EGFP cells were treated with NIH/3T3-LendSN or NIH/3T3-LXSN cells as a control. At the end of the in vivo experiment, plasma Renca-EGFP-sorted cells and tissue lung samples were collected. A real-time PCR array for NF-kappa B target genes revealed that ES therapy led to down regulation of Bcl-3 (P < 0.031), NF-kappa B1 (P < 0.001) and c-Rel (P < 0.004) in the ES-treated group. Using an electrophoretic mobility shift assay (EMSA), we observed a reduction in NF-kappa B binding activity in ES-treated Renca-EGP cells. Furthermore, a supershift assay showed a clear shift of the NF-kappa B DNA band in samples incubated with a p50 antibody. By immunohistochemistry analysis, ES treatment resulted in a significant reduction in expression of p50. (ES vs. control P < 0.05). the immunoprecipitation experiments confirmed the presence of a p50/Bcl-3 complex in nuclear extracts from cells of metastatic lung tissues. Our findings indicate that p50 and Bcl-3 plays a regulatory role in gene transcription in RCC. (C) 2014 Elsevier Masson SAS. All rights reserved.en
dc.description.affiliationUniversidade Federal de São Paulo, Div Nephrol, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Sch Dent, Dept Oral Pathol, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Inst Biomed Sci, Dept Pharmacol, São Paulo, Brazil
dc.description.affiliationIPEN CNEN, Dept Biotechnol, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Div Nephrol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIDFAPESP: 2010/18969-0
dc.description.sponsorshipIDCAPES: 72-71859
dc.format.extent873-879
dc.identifierhttp://dx.doi.org/10.1016/j.biopha.2014.07.008
dc.identifier.citationBiomedicine & Pharmacotherapy. Paris: Elsevier France-editions Scientifiques Medicales Elsevier, v. 68, n. 7, p. 873-879, 2014.
dc.identifier.doi10.1016/j.biopha.2014.07.008
dc.identifier.issn0753-3322
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/38157
dc.identifier.wosWOS:000345001400008
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofBiomedicine & Pharmacotherapy
dc.rightsinfo:eu-repo/semantics/restrictedAccess
dc.rights.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.subjectRenal cell carcinomaen
dc.subjectNF-kappa Ben
dc.subjectBcl-3en
dc.subjectp50en
dc.subjectEndostatinen
dc.titleInvolvement of the NF-kappa B/p50/Bcl-3 complex in response to antiangiogenic therapy in a mouse model of metastatic renal cell carcinomaen
dc.typeinfo:eu-repo/semantics/article
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