C-kit expression in human osteosarcoma and in vitro assays

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dc.contributor.authorMiiji, Luciana Nakao Odashiro [UNIFESP]
dc.contributor.authorPetrilli, Antonio Sergio [UNIFESP]
dc.contributor.authorDi Cesare, Sebastian
dc.contributor.authorOdashiro, Alexandre Nakao [UNIFESP]
dc.contributor.authorBurnier, Miguel Noel Nascente [UNIFESP]
dc.contributor.authorToledo, Silvia Regina Caminada de [UNIFESP]
dc.contributor.authorGarcia, Reynaldo Jesus
dc.contributor.authorAlves, Maria Teresa de Seixas [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionMcGill Univ
dc.contributor.institutionCtr Hosp Afillie Univ Quebec
dc.date.accessioned2018-06-15T17:44:18Z
dc.date.available2018-06-15T17:44:18Z
dc.date.issued2011-01-01
dc.description.abstractBiologic agents targeting oncogenes have encourage researchs trying to correlate the role of tyrosine kinase in the pathogenesis of tumours. Osteosarcoma is a high grade aggressive neoplasm with poor survival. Our aim was to investigate c-kit immunoexpression, its prognostic relevance for patients with osteosarcoma, and the effect of imatinib mesylate (STI571) on proliferation and invasion of the human osteosarcoma cell line. A retrospective immunohistochemical study was performed on archival formalin-fixed paraffin-embedded specimens from 52 patients with high-grade primary osteosarcoma of extremities treated at the Pediatric Oncology Institute (IOP, GRAAC) and archived in the Department of Pathology, Federal University of Sao Paulo. Only pre-chemotherapy specimens were analyzed. Strongly stained cytoplasm and membrane cells were taken as positive. Human osteosarcoma cells from line MG-63 were incubated and the inhibitory effect of imatinib mesylate (STI571) on cell proliferation and invasion was studied. In 24 cases (46.15%), c-kit was expressed by the cells and c-kit-positive tumors exhibited lower necrosis post-chemotherapy. No correlation was found between c-kit expression and overall and disease-free survival. Imatinib mesylate decreased the rates of cell growth of osteosarcoma cells in low doses and invasion in high doses C-kit-positive tumors had worse response to chemotherapy and imatinib mesylate can play a role in blocking or decreasing the rate of growth of osteosarcoma cells, but not the invasive capacity of these neoplastic cells. These data suggested that imatinib mesylate could be a therapeutic target of strategies against osteosarcoma tumors. Further studies are necessary to confirm this indication.en
dc.description.affiliationUniv Fed Sao Paulo, Dept Pathol, Sao Paulo, Brazil
dc.description.affiliationUniv Fed Sao Paulo, IOP, GRAACC, UNIFESP,Dept Pediat, Sao Paulo, Brazil
dc.description.affiliationMcGill Univ, Henry C Witelson Ocular Pathol Lab, Montreal, PQ, Canada
dc.description.affiliationCtr Hosp Afillie Univ Quebec, Dept Pathol, Quebec City, PQ, Canada
dc.description.affiliationUniv Fed Sao Paulo, Dept Orthoped Surg, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Pathol, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, IOP, GRAACC, UNIFESP,Dept Pediat, Sao Paulo, Brazil
dc.description.affiliationUnifespUniv Fed Sao Paulo, Dept Orthoped Surg, Sao Paulo, Brazil
dc.description.sourceWeb of Science
dc.format.extent775-781
dc.identifierhttp://www.ijcep.com/1109006A.html
dc.identifier.citationInternational Journal Of Clinical And Experimental Pathology. Madison: E-century Publishing Corp, v. 4, n. 8, p. 775-781, 2011.
dc.identifier.fileWOS000298346300006.pdf
dc.identifier.issn1936-2625
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/44032
dc.identifier.wosWOS:000298346300006
dc.language.isoeng
dc.publisherE-century Publishing Corp
dc.relation.ispartofInternational Journal Of Clinical And Experimental Pathology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectOsteosarcomaen
dc.subjectc-kiten
dc.subjectimmunohistochemistryen
dc.subjectin vitro assaysen
dc.subjectprognosisen
dc.titleC-kit expression in human osteosarcoma and in vitro assaysen
dc.typeinfo:eu-repo/semantics/article
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