Development of microsatellite markers for the Neotropical endemic Brazilian Guanabara frog, Euparkerella brasiliensis, through 454 shotgun pyrosequencing

dc.contributor.authorFusinatto, Luciana Ardenghi
dc.contributor.authorLopes, Susana
dc.contributor.authorSilva-Ferreira, Alexandre
dc.contributor.authorAlexandrino, João [UNIFESP]
dc.contributor.authorHaddad, Célio Fernando Baptista
dc.contributor.authorRocha, Carlos Frederico Duarte
dc.contributor.authorSequeira, Fernando
dc.contributor.institutionUniversidade do Estado do Rio de Janeiro (UERJ)
dc.contributor.institutionUniv Porto
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionUniv Estadual Paulista
dc.description.abstractThe new-generation 454 GS-FLX Titanium pyrosequencing was used to isolate microsatellite markers for the Brazilian Guanabara frog, Euparkerella brasiliensis, an Atlantic forest endemic species. Three multiplex polymerase chain reaction sets were optimized for genotyping of 11 polymorphic (di- and tetranucleotide) microsatellite markers. Genetic diversity was assessed in 21 individuals from a population (Reserva Ecologica de Guapiacu, REGUA) located in the central region of the Rio de Janeiro State, in Brazil. the mean number of alleles per locus ranged from 3 to 12. Observed and expected heterozygosities ranged from 0.095 to 0.905 and from 0.094 to 0.904, respectively. After using the Bonferroni correction for multiple tests, there was no evidence of linkage disequilibrium between pairs of loci but deviations for Hardy-Weinberg equilibrium were found in 4 loci. We found no evidence for allele dropouts or stuttering, but we detected the presence of null alleles at loci Eb10 and Eb36. These markers will be useful for analyses of fine-scale population structure and determination of relative effects of habitat loss and fragmentation on population genetic variability within species.en
dc.description.affiliationUniv Estado Rio de Janeiro, Dept Ecol, Inst Biol Roberto Alcantara Gomes, BR-20550011 Rio de Janeiro, RJ, Brazil
dc.description.affiliationUniv Porto, Ctr Invest Biodiversidade & Recursos Genet, Vairao, Portugal
dc.description.affiliationUniversidade Federal de São Paulo, Dept Ciencias Biol, Diadema, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias, Dept Zool, Rio Claro, SP, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Ciencias Biol, Diadema, SP, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)
dc.description.sponsorshipIDCNPq: 142823/2009-0
dc.description.sponsorshipIDCNPq: 304791/2010-5
dc.description.sponsorshipIDCNPq: 470265/2010-8
dc.description.sponsorshipIDCAPES: 0378/11-9
dc.description.sponsorshipIDFCT: PTDC/BIA-BEC/105083/2008
dc.description.sponsorshipIDFCT: SFRH/BPD/27134/2006
dc.description.sponsorshipIDFAPESP: 2005/52727-5
dc.description.sponsorshipIDFAPERJ: E-26/102.404.2009
dc.identifier.citationGenetics and Molecular Research. Ribeirao Preto: Funpec-editora, v. 12, n. 1, p. 230-234, 2013.
dc.relation.ispartofGenetics and Molecular Research
dc.rightsAcesso aberto
dc.subjectAtlantic foresten
dc.subjectEuparkerella brasiliensisen
dc.subject454 Sequencingen
dc.titleDevelopment of microsatellite markers for the Neotropical endemic Brazilian Guanabara frog, Euparkerella brasiliensis, through 454 shotgun pyrosequencingen