Lipidomic Assessment of Plasma and Placenta of Women with Early-Onset Preeclampsia

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dc.contributor.authorKorkes, Henri Augusto [UNIFESP]
dc.contributor.authorSass, Nelson [UNIFESP]
dc.contributor.authorMoron, Antonio F. [UNIFESP]
dc.contributor.authorCâmara, Niels Olsen Saraiva [UNIFESP]
dc.contributor.authorBonetti, Tatiana [UNIFESP]
dc.contributor.authorCerdeira, Ana Sofia
dc.contributor.authorCotrim Guerreiro Da Silva, Ismael Dale [UNIFESP]
dc.contributor.authorDe Oliveira, Leandro [UNIFESP]
dc.contributor.institutionUniversidade Federal de São Paulo (UNIFESP)
dc.contributor.institutionSch Matern Vila Nova Cachoeirinha
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionHarvard Univ
dc.date.accessioned2016-01-24T14:38:01Z
dc.date.available2016-01-24T14:38:01Z
dc.date.issued2014-10-17
dc.description.abstractIntroduction: Adipose tissue is responsible for triggering chronic systemic inflammatory response and these changes may be involved in the pathophysiology of preeclampsia.Objective: To characterize the lipid profile in the placenta and plasma of patients with preeclampsia.Methodology: Samples were collected from placenta and plasma of 10 pregnant women with preeclampsia and 10 controls. Lipids were extracted using the Bligh-Dyer protocol and were analysed by MALDI TOF-TOF mass spectrometry.Results: Approximately 200 lipid signals were quantified. the most prevalent lipid present in plasma of patients with preeclampsia was the main class Glycerophosphoserines-GP03 (PS) representing 52.30% of the total lipid composition, followed by the main classes Glycerophosphoethanolamines-GP02 (PEt), Glycerophosphocholines-GP01 (PC) and Flavanoids-PK12 (FLV), with 24.03%, 9.47% and 8.39% respectively. When compared to the control group, plasma samples of patients with preeclampsia showed an increase of PS (p<0.0001), PC (p<0.0001) and FLV (p<0.0001). Placental analysis of patients with preeclampsia, revealed the PS as the most prevalent lipid representing 56.28%, followed by the main class Macrolides/polyketides-PK04 with 32.77%, both with increased levels when compared with patients control group, PS (p, 0.0001) and PK04 (p<0.0001).Conclusion: Lipids found in placenta and plasma from patients with preeclampsia differ from those of pregnant women in the control group. Further studies are needed to clarify if these changes are specific and a cause or consequence of preeclampsia.en
dc.description.affiliationUniversidade Federal de São Paulo, Dept Obstet, São Paulo, Brazil
dc.description.affiliationSch Matern Vila Nova Cachoeirinha, Lab Clin & Expt Invest, São Paulo, Brazil
dc.description.affiliationUniv São Paulo, Dept Immunol, São Paulo, Brazil
dc.description.affiliationUniversidade Federal de São Paulo, Dept Gynecol, São Paulo, Brazil
dc.description.affiliationHarvard Univ, Beth Israel Deaconess Med Ctr, Dept Med, Sch Med, Boston, MA 02215 USA
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Obstet, São Paulo, Brazil
dc.description.affiliationUnifespUniversidade Federal de São Paulo, Dept Gynecol, São Paulo, Brazil
dc.description.sourceWeb of Science
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFoundation for Research Support of the State of São Paulo
dc.description.sponsorshipIDCNPq: 476486/2011-4
dc.description.sponsorshipIDFoundation for Research Support of the State of São Paulo: 12/02270-2
dc.format.extent8
dc.identifierhttp://dx.doi.org/10.1371/journal.pone.0110747
dc.identifier.citationPlos One. San Francisco: Public Library Science, v. 9, n. 10, 8 p., 2014.
dc.identifier.doi10.1371/journal.pone.0110747
dc.identifier.fileWOS000345204100106.pdf
dc.identifier.issn1932-6203
dc.identifier.urihttp://repositorio.unifesp.br/handle/11600/38334
dc.identifier.wosWOS:000345204100106
dc.language.isoeng
dc.publisherPublic Library Science
dc.relation.ispartofPlos One
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleLipidomic Assessment of Plasma and Placenta of Women with Early-Onset Preeclampsiaen
dc.typeinfo:eu-repo/semantics/article
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