Estimulação elétrica na liberação do Peptídeo Relacionado com Gene da Calcitonina (CGRP) e Substância P (SP) em pele de ratos.
Arquivos
Data
2014
Autores
Antunes, Arainy Suély 
Orientadores
Duailibi, Silvio Eduardo
Tipo
Dissertação de mestrado
Título da Revista
ISSN da Revista
Título de Volume
Resumo
Introdução: A disfunção na liberação de neuropeptídeos acarreta
alterações na pele, podendo gerar distúrbios no processo cicatricial e/ou afecções cutâneas. Sendo assim, a liberação de neuropeptídeos é estudada na literatura, relacionando a utilização de agentes eletrofísicos. Objetivo:
Investigar o efeito da estimulação elétrica na liberação de neuropeptídeos
SP e CGRP em pele de ratos. Métodos: Foram utilizados 28 animais
distribuídos em 4 grupos, Grupo Controle (GC): as amostras foram
coletadas após 60 minutos da tricotomia sem estímulo elétrico; Grupo
Sham (GS): após 60min da tricotomia foram colocadas as placas de
eletrodos com esponja umedecida com cloreto de sódio 0,9% e sobreposto
na linha mediana dorsal e dispersivo na região ventral, com o aparelho
desligado por 30minutos; Grupo Estimulação Elétrica com Polo Positivo
(GPP): após 60 minutos da tricotomia, foi realizada a estimulação elétrica
sobre a linha mediana dorsal com polaridade Positiva e o no Grupo
Estimulação Elétrica com polaridade Negativa (GPN): os mesmos
parâmetros utilizados acima, com a mudança, onde o polo negativo passou
para linha mediana dorsal. Ao término da estimulação elétrica, foram
coletadas amostras de pele, submetidas ao Western blotting para análise
dos neuropeptídeos CGRP e SP. Para a análise estatística foi utilizado o
teste de Análise Variância (ANOVA) para identificar a diferença entre os
grupos. Resultado: Não causou diferença significante na liberação de
CGRP e SP na pele de rato. Conclusão: A estimulação elétrica
ultraexcitante não influenciou na liberação de neuropeptídeos, CGRP e SP,
em pele de ratos.
Introduction: A dysfunction in the release of neuropeptides cause changes in the skin, and may cause disturbances in the healing process and/or skin disorders. Therefore the release of neuropeptides has been studied in the literature, connecting the use of electrophysical agents. Objective: To investigate the effect of electrical stimulation on the release of neuropeptides SP and CGRP in rat skin. Methods: 28 animals were randomly divided into 4 groups, Control Group (CG): the samples were collected 60 minutes after shaving without electrical stimulation; Sham Group (SG): after 60 minutes of trichotomy were placed electrodes plates with damp sponge with sodium chloride 0.9% and superimposed on the dorsal midline and dispersive on the ventral region, with the equipment off for 30 minutes; Electrical Stimulation Positive Pole Group (GPP): later 60 minutes of trichotomy, electrical stimulation was performed on the dorsal midline with positive polarity and the Electrical Stimulation Group with negative polarity (NPG): the same parameters used above, with the change where the negative pole was passed into the dorsal midline. At the end of electrical stimulation, skin samples, tested by Western Blotting for analysis of neuropeptides CGRP and SP, were collected. For statistical analysis, analysis of variance (ANOVA) was used to identify differences between the groups. Result: There was a minor release of neuropeptides from the negative and positive polarity, however, not significant. Conclusion: The ultra-exciting electrical stimulation did not influence the release of neuropeptides, SP and CGRP, in rat skin.
Introduction: A dysfunction in the release of neuropeptides cause changes in the skin, and may cause disturbances in the healing process and/or skin disorders. Therefore the release of neuropeptides has been studied in the literature, connecting the use of electrophysical agents. Objective: To investigate the effect of electrical stimulation on the release of neuropeptides SP and CGRP in rat skin. Methods: 28 animals were randomly divided into 4 groups, Control Group (CG): the samples were collected 60 minutes after shaving without electrical stimulation; Sham Group (SG): after 60 minutes of trichotomy were placed electrodes plates with damp sponge with sodium chloride 0.9% and superimposed on the dorsal midline and dispersive on the ventral region, with the equipment off for 30 minutes; Electrical Stimulation Positive Pole Group (GPP): later 60 minutes of trichotomy, electrical stimulation was performed on the dorsal midline with positive polarity and the Electrical Stimulation Group with negative polarity (NPG): the same parameters used above, with the change where the negative pole was passed into the dorsal midline. At the end of electrical stimulation, skin samples, tested by Western Blotting for analysis of neuropeptides CGRP and SP, were collected. For statistical analysis, analysis of variance (ANOVA) was used to identify differences between the groups. Result: There was a minor release of neuropeptides from the negative and positive polarity, however, not significant. Conclusion: The ultra-exciting electrical stimulation did not influence the release of neuropeptides, SP and CGRP, in rat skin.
Descrição
Citação
ANTUNES, Arainy Suély. Estimulação elétrica na liberação do Peptídeo Relacionado com Gene da Calcitonina (CGRP) e Substância P (SP) em pele de ratos. 2014. 81 f. Dissertação (Mestrado em Ciências) - Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, 2014.